This study was carried out in The Ruminant Research Station (Abu Ghraib)/ Department of Animal Resources Research/ Office of Agricultural Research/ Ministry of Agriculture during the period from July 6, 2017 to Feb 3, 2018. Twenty one non pregnant Awassi Turkish ewes were used in the experiment. The age of the animals ranged between 2-5 years old with a weight ranged between 45-70kg. Befor insert CIDR, The animals were examined with ultrasonography to be sure that they were not pregnant. These ewes were divided into three equal groups of 7 ewes/ treatment. They were fed naturally on basal diet. The first group were not treated (T1) which serve Control group, and injected with 5-10 ml of normal saline in intramuscular. Ewes of Second group were Treated (T2) by injected with a 155 μmol/kg arginine I.V. The Third group were Treated (T3) by injected with 155μmol/kg arginine intramuscular. All Treated ewes were inseminated by rams for 7 days. The inseminate rams were revolved among folds to get rid of their effects on ewes, Each inseminated ram was given a number. Blood samples were taken from jugular vein on the 1st and 2nd days of arginine injection and then insert CIDR and on the 3rd, 5th, 7th, 10th,13th ,and 16th days of this treatment to measure the change in the levels of a blood and chemical properties during arginine treatment. The results of a lab. analysis during the treatment of amino acid arginine showed no significant differences between the treated group with arginine as compared to the control group in the percentages of white blood cells, but there were a differences in the number of Neutrophils in the group of intravenous injection, the group of intramuscular injection, (75.14, 75.00), respectively as compared with control group (71.0). Lymphocytes showed no significant differences between parameters under study as well as for Eosinophil. The differences were observed between the coefficients and the study periods. The period (4) was significantly superior (P<0.05) in the arginine treated as Compared with the control group, (20.42 ± 2.61) and the percentage of intravenous injection (17.28±1.98), respectively compared with a control group (19.00 ±2.51), as for Basophil, there was a gradual decrease in all study groups. The results showed that the value of cholesterol a significant superiority (P<0.05) in the treated group with arginine in ewes serum as compared with the control group. It was noticed that the group of intravenous injection of arginine significantly increase the group of intramuscular injection of arginine (P<0.05) (6, 8) in blood cholesterol level. The periods (1, 2, 3, 4, 5, and 7) of injection of amino acid arginine showed no significant differences between them. The glucose concentration level, showed no significant differences between the arginine amino acid treatment group and the control group and at the different. It was concluded from this study that treatment with amino acid arginine did not affect characteristics of the blood, and its chemical properties. |