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Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs Medium | ||
| Basrah Journal of Veterinary Research | ||
| Volume 23, Issue 3, September 2024, Pages 55-62 PDF (536.1 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvr.2024.147966.1071 | ||
| Authors | ||
| Abdul-Samad Uleiwi Hassan1; jian ibraheem* 2 | ||
| 1Al-Forat Al-Awsat Technical University, Health & Medical Techniques College, Medical Laboratories Techniques Department. | ||
| 2Middle Technical University/ Health & Medical Techniques College, Anaesthesia Techniques Department | ||
| Abstract | ||
| This project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes contains m-PBS prelude for incubation by modified in vitro technique 2000, which supplemented with 5% Co2 gas state for 26 hour at 37oc. The gained consequences led to 20% maturation occurred for the second metaphase after applying m-PBS and staining with orcein stain. Statistics supports our hypothesis. The outcomes for this study is that incubation of B. indicus oocytes in vitro by modified technique 2000 with using m-PBS culture media will established a success and promising in maturation rates. This project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes contains m-PBS prelude for incubation by modified in vitro technique 2000, which supplemented with 5% Co2 gas state for 26 hour at 37oc. The gained consequences led to 20% maturation occurred for the second metaphase after applying m-PBS and staining with orcein stain. Statistics supports our hypothesis. The outcomes for this study is that incubation of B. indicus oocytes in vitro by modified technique 2000 with using m-PBS culture media will established a success and promising in maturation rates. | ||
| Keywords | ||
| B. indicus; oocytes; in-vitro maturation; Co2 gas stage; m-PBS | ||
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