Detection of MC4R Gene (c.944 C<T) SNP and it Relationship with productive Performance and Carcass traits in Ross 308

Al-kaledi, Alaa Mohammad-faeq

doi:10.58928/ku24.15413

	Detection of MC4R Gene (c.944 C
Kirkuk University Journal for Agricultural Sciences (KUJAS)
Volume 15, Issue 4, December 2024, Pages 112-119 PDF (519.73 K)
Document Type: Research Paper
DOI: 10.58928/ku24.15413
Author
Alaa Mohammad-faeq Al-kaledi^*
university of Baghdad
Abstract
The objective of this study was to detect the effects of genotypes of MC4R gene in broiler chicken Ross 308. And its relationship with growth and carcass traits. studying the genetic polymorphism of this gene and determining its genotypic to find the relationship between this gene and economic traits in broilers. weekly measurements were taken (the body weight – weight gain -body length – breast circumference - breast depth - leg length - drumstick length – drumstick circumference). Blood samples were collected from 100 bird (21 days old age), and at 42-day carcass measurements were taken for each bird individually, then process of extracting DNA from blood samples was performed and primer for MC4R gene – foreword and revers were used and PCR technic was used to amplified DNA copies in order to for the determining the locations of quantitative traits and determine the single nucleotide polymorphisms (SNP) sequencing of nitrogenous bases. The results show that Genetic variant within MC4R gene were screened through DNA sequencing methods. Molecular Detection showed a SNP at position (c.944C>T), and detected two alleles and three genotypes (CC,CT,TT). A highly significant differences (p<0.01) for CC and TT genotype in body weight at 5th and 6th wk, and in weight gain at 6th wk, and in breast circumference at 3rd wk, and in drumstick weight, and was produced significantly (p<0.05) in breast depth trait at initial, 2nd and 6 wk , and in leg length at 6th wk, and in carcass weight, back weight, abdominal fat.
Keywords
MC4R Gene; ROSS 308; SNP; Growth traits; carcass traits; mutation

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