Bneed, Q., Faja, O. (2024). Phylogenetic analysis and molecular identification of Staphylococcus species isolated from cheese. , 23(1), 35-40. doi: 10.29079/qjvms.2024.185130
Qasim Zamel Bneed; Orooba Meteab Faja. "Phylogenetic analysis and molecular identification of Staphylococcus species isolated from cheese". , 23, 1, 2024, 35-40. doi: 10.29079/qjvms.2024.185130
Bneed, Q., Faja, O. (2024). 'Phylogenetic analysis and molecular identification of Staphylococcus species isolated from cheese', , 23(1), pp. 35-40. doi: 10.29079/qjvms.2024.185130
Bneed, Q., Faja, O. Phylogenetic analysis and molecular identification of Staphylococcus species isolated from cheese. , 2024; 23(1): 35-40. doi: 10.29079/qjvms.2024.185130

Phylogenetic analysis and molecular identification of Staphylococcus species isolated from cheese

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 23, Issue 1, May 2024, Pages 35-40    PDF (247.04 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2024.185130
Authors
Qasim Zamel Bneed* ; Orooba Meteab Faja
Department of Public Health, College of Veterinary, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq
Abstract
The purpose of the study was to identify and characterize the microbes found in the cheese samples that were collected from various Al-Diwaniyah City markets using molecular methods. In Al-Diwaniyah City, 75 cheese samples were collected from three different sources. The samples were cultured on the culture media. Detection of the isolated bacteria were done by using the staining, the culture, and the biochemical examination such as catalase tests, as well as PCR. 75 cheese samples tested positive for S. aureus 37(48%), S. epidermidis 20 (26.6%) and S. intermedius 18(24%). A 16S rRNA gene was used to amplify twenty isolates. All isolates were identified by PCR by using 16S rRNA gene and DNA sequencing. All of the examined isolates showed similarity of (93-99) % to those in the Gen Bank of NCBI.
Keywords
cheese; Staphylococcus Spp; gene 16s rRNA; Phylogenetic analysis
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