The activity of three different propolis extracts and their antioxidant activity induced by CCL4 in adult rats

Hasan, Rosa M.; Ghazi, Ali Mohammed

doi:10.29079/qjvms.2024.185132

	The activity of three different propolis extracts and their antioxidant activity induced by CCL4 in adult rats
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 23, Issue 1, May 2024, Pages 46-56 PDF (342.72 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2024.185132
Authors
Rosa M. Hasan¹; Ali Mohammed Ghazi^* ²
¹Unit of Zoonotic Diseases, College of Veterinary medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.
²Unit of zoonotic disease / college of veterinary medicine/ University of Al-Qadisiyah
Abstract
The goal of the current study was to examine the impact of extracting propolis in various solvents in light of the antioxidant activity induced by CCL4. The biological study was conducted using 25 adult Wistar male rats. Five rats served as normal control group which fed on basal diet with drinking tap water. While, other 20 rats received intra-peritoneal single dose of 0.5 ml/kg CCl4 to induce renal and hepatic oxidative stress. Then, rats reclassified into 4 groups (n=5), positive control and the other three groups were treated by watery, ethanolic and ethyl acetate extracts of propolis instead of water till the end of experimental period (9 days). In vivo extracts evaluation includes serum Catalase, SOD, GSH, TAOC, and MDA were measured at the end of the experiment. The In vitro results showed all extracts exhibited increasing radical scavenging activities with increased concentrations of extract with variation in antioxidant activity according to the type of extract. The CCL4 group rats showed significant (P<0.05) decline in the catalase, SOD, GSH and TAOC and significant (P<0.05) evaluation in the MDA compared with control. All prepared extracts corrected the deviation occurring in the parameters as a result of administering the CCL4, with variation depending on the type of extract. Watery extract showed best results in enhance catalase level, while ethanolic extract showed best results in enhance GSH activity, no difference in the SOD, T-AOC and MDA between watery and ethanolic extracts, in the same time the ethyl acetate extract was less effective. The In vitro and In vivo antioxidant activity was varied according to the type of extract; ethanol gave best activity.
Keywords
Propolis; extracts; antioxidants; CCL4

References
1-Abutaha, Nael, AL-Zharani, Mohammed, Alotaibi, Amal, Cordero, Mary Anne W., Bepari, Asmatanzeem and Alarifi, Saud. (2021). In vitro and in vivo investigation of polypharmacology of propolis extract as anticancer, antibacterial, anti-inflammatory, and chemical properties. Open Chemistry, vol. 19, no. 1, 2021, pp. 864-874. DOI.org/10.1515/chem-2021-0076 https://doi.org/10.1515/chem-2021-0076 2-Bankova, V., Popova, M., (2007). Propolis of stingless bees: a promising source of biologically active compounds. Pharmacogn. Rev. 1, 88-92. DOI.org/10.1016/j.jff.2021.104902. 3-Wagh, V. D. (2013). Propolis: A Wonder Bees Product and Its Pharmacological Potentials. Advances in Pharmacological Sciences, 2013, 1-11. DOI:10.1155/2013/308249 https://doi.org/10.1155/2013/308249 4-Bankova, V., (2000). Determining quality in propolis samples. J. Am. Apither Soc; Vol 7,N. 2 . www.apitherapy.org/determining. 5-Pietta, P.; Gardana, A. and Pietta, A. (2012). Analytical methods for quality control of propolis. Fitoterapia. S7-S20. DOI: 10.1016/s0367-326x(02)00186-7.https://doi.org/10.1016/S0367-326X(02)00186-7 6-Sherif, S.; Rehab, T. and Hamdia, A.(2016). GC/MS characterization of Egyptian propolis different extracts. J. of scientific Research in Sicence. Vol.23. 239-246.DOI: 10.21608/JSRS.2016.15634 https://doi.org/10.21608/jsrs.2016.15634 7-Forma, E., & Bryś, M. (2021). Anticancer Activity of Propolis and Its Compounds. Nutrients, 13(8), 2594. DOI.org/10.3390/nu13082594https://doi.org/10.3390/nu13082594 8-Ghazi A.M, and Al-Bayati M. Metabolomic profiling of Iraqi propolis Samples Collected from Al-Diwanyiah city. QJVMS (2019) Vol. 18 No. (2):25-34. 9-Mouse, H. A., Tilaoui, M., Jaafari, A., M'barek, L. A., Aboufatima, R., Chait, A., & Zyad, A. (2012). Evaluation of the in vitro and in vivo anticancer properties of Moroccan propolis extracts. Revista Brasileira de Farmacognosia, 22(3), 558-567. DOI.org/10.1590/S0102-695X2012005000030https://doi.org/10.1590/S0102-695X2012005000030 10- Fahad M, AL-Shemmmari W. Inovo injection of water extracted propolis on hatchability and some physiological characteristics of broiler chickens. QJVMS (2013) Vol. 12 No. (1): 45-51. 11-Marjoni M, and Zulfisa,A. (2017). Antioxidant Activity of Methanol Extract/Fractions of Senggani Leaves (Melastoma candidum D. Don). Pharmaceutica Analytica Acta, 08(08). DOI: 10.4172/2153-2435.1000557.https://doi.org/10.4172/2153-2435.1000557 12-Herrera-Calderon O, Alvarado-Puray C, Arroyo-Acevedo JL, Rojas-Armas JP, Chumpitaz-Cerrate V, Hañari-Quispe R, et al. (2021). Phytochemical screening, total phenolic content, antioxidant, and cytotoxic activity of five peruvian plants on human tumor cell lines. Phcog Res;10:161-5. DOI:10.4103/pr.pr_109_17.https://doi.org/10.4103/pr.pr_109_17 13-Chaouche, T. M., Haddouchi, F., Ksouri, R., & Atik-Bekkara, F. (2014). Evaluation of antioxidant activity of hydromethanolic extracts of some medicinal species from South Algeria. Journal of the Chinese Medical Association, 77(6), 302-307. DOI.org/10.1016/j.jcma.2014.01.009https://doi.org/10.1016/j.jcma.2014.01.009 14-Sarma, R., Das, M., Mudoi, T., Sharma, K. K., Kotoky, J., & Devi, R. (2016). Evaluation of Antioxidant and Antifungal Activities of Polyphenol-rich Extracts of Dried Pulp of Garcinia morella Gaertn. Tropical Journal of Pharmaceutical Research January 2016; 15 (1): 133-140. DOI:10.4314/tjpr.v15i1.19https://doi.org/10.4314/tjpr.v15i1.19 15-Al-Harbi, N. O., Imam, F., Nadeem, A., Al-Harbi, M. M., Iqbal, M., & Ahmad, S. F. (2014). Carbon tetrachloride-induced hepatotoxicity in rat is reversed by treatment with riboflavin. International Immunopharmacology Volume 21, Issue 2, August 2014, Pages 383-388. https://DOI.org/10.1016/j.intimp.2014.05.014.https://doi.org/10.1016/j.intimp.2014.05.014 16-Al-Rubii, A.K. (2018). Effect of Tribulus terrestris L. extract on prostatic and testicular functions of castrated rabbits. M.Sc thesis. College of science. Baghdad University. Baghdad. Iraq. https://doi.org/10.30539/iraqijvm.v36i2.499 17-Daniel, W. (2009). Biostatistics: A Foundation for Analysis in the Health Sciences. 9th edition. John Wiley and Sons. INC. USA. 18-Boufadi, Y. Soubhye, J. Riazi, A. Rousseau, A. Vanhaeverbeek, M. Nève, J. Boudjeltia, K. Van Antwerpen. P. (2014). Characterization and antioxidant properties of six Algerian propolis extracts: ethyl acetate extracts inhibit myeloperoxidase activity Int. J. Mol. Sci., 15, pp. 2327-2345. DOI.org/10.3390/ijms15022327https://doi.org/10.3390/ijms15022327 19-Koo, H., Rosalen, P. L., Cury, J. A., Ambrosano, G. M., Murata, R. M., Yatsuda, R., ... & Park, Y. K. (2000). Effect of a new variety of apis mellifera propolis onmutans streptococci. Current microbiology, 41, 192-196.‏ https://doi.org/10.1007/s0028400101170 20- Kosalec, Ivan & Bakmaz, Marina & Pepeljnjak, Stjepan & Vladimir-Knežević, Sanda. (2004). Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta pharmaceutica (Zagreb, Croatia). 54. 65-72. Corpus ID: 5691293 21-Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, Gargiulo G, Testa G, Cacciatore F, Bonaduce D, Abete P. Oxidative stress, aging, and diseases. Clin Interv Aging. 2018 Apr 26;13:757-772. https://doi.org/10.2147/CIA.S158513 22-Luo J, Mills K, le Cessie S, Noordam R, van Heemst D. Ageing, age-related diseases and oxidative stress: What to do next? Ageing Res Rev. 2020 Jan;57:100982. DOI: 10.1016/j.arr.2019.100982. https://doi.org/10.1016/j.arr.2019.100982 23-Checa J, Aran JM. Reactive Oxygen Species: Drivers of Physiological and Pathological Processes. J Inflamm Res. 2020 Dec 2;13:1057-1073. https://doi.org/10.2147/JIR.S275595 24-Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., … Bitto, A. (2017). Oxidative Stress: Harms and Benefits for Human Health. Oxidative Medicine and Cellular Longevity, 2017, 1-13. https://doi.org/10.1155/2017/8416763 25-Yosri N, Abd El-Wahed AA, Ghonaim R, Khattab OM, Sabry A, Ibrahim MAA, Moustafa MF, Guo Z, Zou X, Algethami AFM, Masry SHD, AlAjmi MF, Afifi HS, Khalifa SAM, El-Seedi HR. (2021). Anti-Viral and Immunomodulatory Properties of Propolis: Chemical Diversity, Pharmacological Properties, Preclinical and Clinical Applications, and In Silico Potential against SARS-CoV-2. Foods. 31;10(8):1776. https://doi.org/10.3390/foods10081776 26-Da cruz, F., Martins, D.; Ferreira, J.; Magalhaes,P.; Silveria, D.; Bazzo, Y. (2022). Antioxidant activity of apis mellifera bee propolis: A review. J. of natural products discovey. Volume 1, Issue 1, DOI 10.24377/jnpd.article655. 27-Benhanifia, M., Mohamed, W. M., Bellik, Y., & Benbarek, H. (2013). Antimicrobial and antioxidant activities of different propolis samples from north-western Algeria. International Journal of Food Science & Technology, 48(12), 2521-2527. https://doi.org/10.1111/ijfs.12244 28-Ma, S. & Ma, Haile & Pan, Zhongli & Luo, Lin & Weng, L.. (2016). Antioxidant activities of propolis's extracts by different solvents in vitro. 16. 53-58. DOI:10.16429/j.1009-7848.2016.08.008 29- El-Haskoury R, Al-Waili N, Kamoun Z, Makni M, Al-Waili A, Lyoussi B. Antioxidant activity and protective effect of propolis against carbon tetrachloride-induced liver and kidney injury by modulation of oxidative parameters. Vet World. 2021 Dec;14(12):3076-3083. https://doi.org/10.14202/vetworld.2021.3076-3083 30-Magnavacca A, Sangiovanni E, Racagni G, Dell'Agli M. The antiviral and immunomodulatory activities of propolis: An update and future perspectives for respiratory diseases. Med Res Rev. 2022 Mar;42(2):897-945. https://doi.org/10.1002/med.21866
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