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Silver Nanoparticle Impact as Antibacterial and Antibiofilm Agent Against MDR K.pneumonaie in Mosul City | ||
| Annals of the College of Medicine, Mosul | ||
| Volume 46, Issue 2, December 2024, Pages 159-169 PDF (757.17 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/mmed.2024.146648.1253 | ||
| Authors | ||
| Zena Makkie Alyouzbakee* 1; Khalid Omar Mohammad2 | ||
| 1Microbiology department/Mosul University/Medical College | ||
| 2Microbiology department/ Tikrit University/Medical College | ||
| Abstract | ||
| The prevalence of antibiotic resistance continues to rise rapidly, necessitating the development of innovative approaches to combat diseases caused by drug-resistant bacteria. One of these approaches is pharmaceutical nanotechnology, which has the potential to generate novel therapeutic compounds for biomedical applications. Of these substances, silver nanoparticles (AgNPs) exhibit promising antibacterial and anti-biofilm properties. Two hundred and thirty different clinical samples (urine, sputum, diabetic foot pus, pleural fluid, tracheostomy tube, cerebrospinal fluid, folly's catheter, ear swab, and blood) were collected looking for K.pneumonaie, which are multiple drug-resistant (MDR). Twenty-three K.pneumonaie clinical isolates were identified by conventional bacteriological media and confirmed by the VITEK-2GN system. Then, the biofilm-forming activity was assessed using qualitative methods such as Congo Red agar and quantitative methods such as microtiter plate assay that involved crystal violet staining. Conventional PCR detected fimH and mrkD biofilm gene frequency among biofilm-producing K.pneumonaie. The chemically synthesized AgNPs were used, and their Minimal Inhibitory Concentration (MIC) and Minimal Biofilm Inhibition Concentration (MIBC) were determined. Biofilm genes, namely fimH and mrkD gene, had been detected before and after treatment with silver nanoparticles in the clinical isolates. The findings indicated that all isolates resisted the tested antibiotics and could build biofilms to various degrees (19 had strong biofilm capability,3 were moderate biofilm producers, and only 1 had weak biofilm production capability). The susceptibility of all isolates to AgNPs was demonstrated by their antibacterial activity, with MIC and MIBC values being 50000 μg/ml or 25000 μg/ml according to the isolates. In addition, applying AgNPs resulted in a substantial decrease in biofilm formation, with 8 out of 23 isolates converting to non-biofilm producers. Biofilm genes fimH and mrkD were present in 100% and 78.26%, respectively, and not affected by silver nanoparticle treatment. There was a synergistic effect between silver nanoparticles and imipenem in compacting MDR K.pneumonaie. | ||
| Keywords | ||
| Silver nanoparticle; MDR K.pneumonaie; Minimal Inhibitory Concentration; Minimal Biofilm Inhibition Concentration; biofilm genes | ||
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