Efficacy of Silicone Gel Plus Thrombin Adhesives on Cutaneous 2nd Degree Burn Healing

Hussein, Noor K.; Towfik, Amir I.

doi:10.29079/qjvms.2024.185610

	Efficacy of Silicone Gel Plus Thrombin Adhesives on Cutaneous 2nd Degree Burn Healing
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 23, Issue 2, December 2024, Pages 18-28 PDF (1.24 M)
Document Type: Research Paper
DOI: 10.29079/qjvms.2024.185610
Authors
Noor K. Hussein^* ¹; Amir I. Towfik²
¹Department of Surgery and Obstetric, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
²Department of Surgery and Obstetric/College of Veterinary Medicine/ University of Al-Qadisiyah, Iraq.
Abstract
A lot of researchers try many types of therapies for burns healing in Veterinary medicine in the last decades. Silicone gel may decrease the scar tissue, while thrombin may increase the proliferation of cells. This project aims to compare the efficacy of silicone gel combined and thrombin on 2^nd degree cutaneous burn healing. Forty female white albino rats, who were housed in plastic boxes divided randomly into four groups 10 rats for each. Control group G1 had no treatment, second group G2 treated with silicone gel, third group treated with autologous thrombin adhesive, fourth group treated with combination of silicone gel plus autologous thrombin adhesive. Under routine surgery with general anesthesia all rats had thermal injuries at their backs. The thermal injuries were then created using a solid aluminum bar with a diameter of 2 cm in diameter had previously been heated to 100°C in boiling water. The bar is kept in touch with the rat's skin in the dorsal proximal region of the back. Hyperemia, edema, scar tissue, and scab were recorded for morphological exam. The therapies were applied topically once daily for 10 days. At the time of burn and on the appropriate days for the biopsies at 7^th ,14^th,21^th, and 28^th day, sterile "swabs" were taken and sent to laboratory for bacterial contamination. Biopsies were collected for histopathologic evaluation of healing. The morphologic appearance was recorded at 1 day, 7^th day, 14^th day,21^th day, and 28^th day, it was the best in G3. A completed wound healing was recorded highly significant wound contraction in G3 groups. The bacterial colonies count was recorded the lowest in G3. Histopathologic evaluation showed the superiority of thrombin adhesive than silicone gel for 2^nd degree burns healing and this result accompanied with the other parameters. Histological changes post burn injury in thrombin treatment showed complete healing of all skin layers including epidermis, papillary dermis, reticular dermis, and hypodermis, hair follicles, sweat glands, and sebaceous glands were obvious while in silicone gel showed regenerated epidermis layer, gaps between epidermis and dermis, edema or bulla like lesion between epidermis and dermis, subcutaneous edema, and infiltration of inflammatory cell in the dermis mostly lymphocytes.E. The study concluded that silicone gel decreases scar formation and thrombin accelerate burn healing.
Keywords
silicone; gel; thrombin; adhesive; cutaneous; burn; healing

References
1. Garcia VG, de Lima MA, Okamoto T, Milanezi LA, Júnior ECG, Fernandes LA, et al. Effect of photodynamic therapy on the healing of cutaneous third-degree burn: histological study in rats. Lasers Med Sci. 2010;25(2):221-8.https://doi.org/10.1007/s10103-009-0694-z 2. Bayat M, Vasheghani MM, Razavi N. Effect of low-level helium-neon laser therapy on the healing of third-degree burns in rats. J Photochem Photobiol B. 2006;83(2):87-93.https://doi.org/10.1016/j.jphotobiol.2005.12.009 3. Alsarhan M, Hussein A. The healing effect of Chitosan nanomaterial versus Low-Level Laser Therapy (660nm) on Second Degree Burn Wounds in Rats. QJVMS. 2023;22(1):46-52. 4. Mustoe TA. Evolution of silicone therapy and mechanism of action in scar management. Aesthetic Plast Surg. 2008;32(1):82-92.https://doi.org/10.1007/s00266-007-9030-9 5. Monstrey S, Middelkoop E, Vranckx JJ, Bassetto F, Ziegler UE, Meaume S, et al. Updated scar management practical guidelines: non-invasive and invasive measures. J Plast Reconstr Aesthet Surg. 2014;67(8):1017-25.https://doi.org/10.1016/j.bjps.2014.04.011 6. Perkins K, Davey RB, Wallis KA. Silicone gel: a new treatment for burn scars and contractures. Burns. 1983;9(3):201-4.https://doi.org/10.1016/0305-4179(83)90039-6 7. Düregger K, Frenzel S, Eblenkamp M. Autologous fibrin glue: automated production and adhesive quality. Curr Dir Biomed Eng. 2017;3(2):397-400.https://doi.org/10.1515/cdbme-2017-0083 8. Galante R, Pinto TJ, Colaco R, Serro AP. Sterilization of hydrogels for biomedical applications: A review. J Biomed Mater Res B Appl Biomater. 2018;106(6):2472-92.https://doi.org/10.1002/jbm.b.34048 9. Kamoun EA, Chen X, Eldin MSM, Kenawy ERS. Crosslinked poly(vinyl alcohol) hydrogels for wound dressing applications: A review of remarkably blended polymers. Arab J Chem. 2015;8(1):1-14.https://doi.org/10.1016/j.arabjc.2014.07.005 10. Patil PP, Reagan MR, Bohara RA. Silk fibroin and silk-based biomaterial derivatives for ideal wound dressings. Int J Biol Macromol. 2020;164:4613-27.https://doi.org/10.1016/j.ijbiomac.2020.08.041 11. Akrami-Hasan-Kohal M, Tayebi L, Ghorbani M. Curcumin-loaded naturally-based nanofibers as active wound dressing mats: Morphology, drug release, cell proliferation, and cell adhesion studies. New J Chem. 2020;44(25):10343-51.https://doi.org/10.1039/D0NJ01594F 12. Ahn ST, Monafo WW, Mustoe TA. Topical silicone gel for the prevention and treatment of hypertrophic scar. Arch Surg. 1991;126(4):499-504.https://doi.org/10.1001/archsurg.1991.01410280103016 13. Buchanan MR, Ofosu FA, Brister SJ. Thrombin: Its key role in thrombogenesis-Implications for its inhibition. CRC Press. 14. Dhivya S, Padma VV, Santhini E. Wound dressings-a review. BioMedicine. 2015;5(4):22.https://doi.org/10.7603/s40681-015-0022-9 15. Patterson C, Stouffer GA, Madamanchi N, Runge MS. New tricks for old dogs: nonthrombotic effects of thrombin in vessel wall biology. Circ Res. 2001;88(10):987-97.https://doi.org/10.1161/hh1001.091447 16. Lawson JH. The clinical use and immunologic impact of thrombin in surgery. Semin Thromb Hemost. 2006;32(S1):98-110.https://doi.org/10.1055/s-2006-939559 17. Wagner EH, Austin BT, Von Korff M. Organizing care for patients with chronic illness. Milbank Q. 1996;74(4):511-44.https://doi.org/10.2307/3350391 18. Mishra SK, Mary DS, Kannan S. Copper incorporated microporous chitosan-polyethylene glycol hydrogels loaded with naproxen for effective drug release and anti-infection wound dressing. Int J Biol Macromol. 2017;95:928-37.https://doi.org/10.1016/j.ijbiomac.2016.10.080 19. Bjarnsholt T, Kirketerp‐Møller K, Jensen PØ, Madsen KG, Phipps R, Krogfelt K, et al. Why chronic wounds will not heal: a novel hypothesis. Wound Repair Regen. 2008;16(1):2-10.https://doi.org/10.1111/j.1524-475X.2007.00283.x 20. Tan EM, Rouda S, Greenbaum SS, Moore JH Jr, Fox J, Sollberg S. Acidic and basic fibroblast growth factors down-regulate collagen gene expression in keloid fibroblasts. Am J Pathol. 1993;142(2):463. 21. Ricketts CH, Martin L, Faria DT, Saed GM, Fivenson DP. Cytokine mRNA changes during the treatment of hypertrophic scars with silicone and nonsilicone gel dressings. Dermatol Surg. 1996;22(11):955-9.https://doi.org/10.1111/j.1524-4725.1996.tb00640.x 22. Tan EM, Rouda S, Greenbaum SS, Moore JH Jr, Fox J, Sollberg S. Acidic and basic fibroblast growth factors down-regulate collagen gene expression in keloid fibroblasts. Am J Pathol. 1993;142(2):463. 23. Ricketts CH, Martin L, Faria DT, Saed GM, Fivenson DP. Cytokine mRNA changes during the treatment of hypertrophic scars with silicone and nonsilicone gel dressings. Dermatol Surg. 1996;22(11):955-9.https://doi.org/10.1111/j.1524-4725.1996.tb00640.x
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