Al-Zamely, H., Namaa, N. (2024). Comparative study between the effect of Ginseng extract and Ginseng nanoparticles using SDHA gene expression as an indicator for mitochondrial function in male rats. , 23(2), 37-40. doi: 10.29079/qjvms.2024.185612
Hayder Abdulkadem Al-Zamely; Noor Abbas Namaa. "Comparative study between the effect of Ginseng extract and Ginseng nanoparticles using SDHA gene expression as an indicator for mitochondrial function in male rats". , 23, 2, 2024, 37-40. doi: 10.29079/qjvms.2024.185612
Al-Zamely, H., Namaa, N. (2024). 'Comparative study between the effect of Ginseng extract and Ginseng nanoparticles using SDHA gene expression as an indicator for mitochondrial function in male rats', , 23(2), pp. 37-40. doi: 10.29079/qjvms.2024.185612
Al-Zamely, H., Namaa, N. Comparative study between the effect of Ginseng extract and Ginseng nanoparticles using SDHA gene expression as an indicator for mitochondrial function in male rats. , 2024; 23(2): 37-40. doi: 10.29079/qjvms.2024.185612

Comparative study between the effect of Ginseng extract and Ginseng nanoparticles using SDHA gene expression as an indicator for mitochondrial function in male rats

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 23, Issue 2, December 2024, Pages 37-40    PDF (264.17 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2024.185612
Authors
Hayder Abdulkadem Al-Zamely* ; Noor Abbas Namaa
Department of Physiology, Biochemistry, and Pharmacology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq
Abstract
The current research was conducted to explore the feasibility of employing ginseng nanoparticles (NPs) to improve sperm mitochondrial functions in vivo. For this purpose, a total of (60) adult male Wister rats were explored, acclimated at the animal house of the College of Veterinary Medicine at Al-Qadisiyah University. They prepared and divided into three equal groups. T1, T2, and control negative received only distilled water daily and no other treatment. T1 was given a daily dose of 500 mg/kg of ginseng extract orally by stomach tube. While T2 received orally a daily dose of 250 mg/kg of ginseng NPs by stomach tube. After a 60-day experimental period, all study animals were put to anesthesia and dissection to collect testis samples that were subjected to gene expression (real-time RT, polymerase chain reaction PCR) analysis through targeting Succinate dehydrogenase complex flavoprotein subunit A (SDHA) genes. The results of calculating the gene expression of the gene SDHA showed a significant (P<0.05) increase in the level of SDHA gene expression in both the group of rats treated with the Ginseng extract (13.03±1.49) and the group of rats treated with ginseng nanoparticles (48.46±3.85), compared with the negative control group of rats(1±0C). At the same time, the study results showed a significant (P<0.05) increase in the level of gene expression in the group of rats treated with nanoparticles when compared with the group of rats treated with extract only. Using sperm analysis and a molecular biologic approach, this study conformal the impact of ginseng on spermatogenesis in rat testes.
Keywords
SDHA gene; testes; nanoparticles (NPs); gene expiration (qPCR); Ginseng
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