Efficacy of Lyophilized Urinary Bladder submucosa on open wounds healing

Kadhim, Sameer S.; Hamza, Abdulsatar S.

doi:10.29079/qjvms.2024.185633

	Efficacy of Lyophilized Urinary Bladder submucosa on open wounds healing
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 23, Issue 2, December 2024, Pages 41-47 PDF (617.29 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2024.185633
Authors
Sameer S. Kadhim^* ; Abdulsatar S. Hamza
Department of Surgery and Obstetric / College of Veterinary Medicine /University of Al-Qadisiyah, Iraq
Abstract
The study's objective was to assess the effectiveness of Lyophilized Urinary Bladder Submucosa in the healing of open wounds. Study subjects included 60 mature, white New Zealand rabbits. All of the animals were housed in same bedding and environmental circumstances. They divided into two groups (n = 30). After general anesthesia and prepared for aseptic surgery, a punch used to make circular full-thickness (1.5 cm) skin incision on the backs of the animals. The rabbits were sorted into two equal groups at random. Histopathological specimens were collected at 7, 14, and 21 days after wounding. Group 1 served as a control group for untreated wounds. Lyophilized bovine UBM was used as a scaffold in Group 2 to treat wound on the first day following surgery, whereas 2gm of UBM hydrogel was applied topically once daily from the second day for 7 days. Fresh urinary bladder was used to form UBM as a lyophilized scaffold and hydrogel was made for treatment of group 2. Due to the hyperplasia of the epidermal layers. In the dermis, tiny collagen fibers replace the injured tissue and encourage the growth of new blood vessels and inflammatory cells. At day 21, G2 demonstrated the development of a keratinized layer over the epidermis. The epidermal layers seemed hyperplastic, and the two skin wound edges appeared to be linked. The dermis has a network of fibrosis and collagen that is regularly arranged and new blood vessels. Additionally, there were fresh, tiny hair follicles in the area of the wound.
Keywords
Lyophilized; Urinary Bladder; submucosa; wound; healing

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