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ISOLATION, PURIFICATION AND CHARACTERIZATION OF EXTRACELLULAR MYCOTOXIN FROM Alternaria alternata | ||
| Basrah Journal of Veterinary Research | ||
| Volume 8, Issue 1, 2009, Pages 176-189 PDF (317.69 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvr.2009.185671 | ||
| Abstract | ||
| The isolate of Alternaria alternata that isolated from soil produced extracellular secondary metabolites (Mycotoxin) of carbohydrate with reducing nature which had inhibitory effect against Gm positive & Gm negative standard and pathogenic bacteria in solid media. Three types of media Malt Extract Agar (MEA), Potato Dextrose Agar (PDA) and Potato Carrot Agar (PCA) can be using as solid or liquid media for production this mycotoxin. The mycotoxin extracted from free cell supernatant by simple extraction method using ethanol 90%. The cytotoxicity of extracted mycotoxin against human blood cells (RBCs) was (25) ppm. The acute toxicity of extracted toxin as determined by LD50 of laboratory mice was 150 mg/kg. | ||
| Keywords | ||
| Mycotoxin; Alternaria; PDA | ||
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