Molecular Diagnosis Of The Pothos Plant Epipremnum aureum Cultivated In Nineveh Governorate For The First Time In Iraq

Ibrahim, Shahad Faris; AL-Hatem, Jahan Yahya; AL-Taee, Anwar Fakhre

doi:10.33899/berj.2024.185735

	Molecular Diagnosis Of The Pothos Plant Epipremnum aureum Cultivated In Nineveh Governorate For The First Time In Iraq
College Of Basic Education Research Journal
Article 31, Volume 20, Issue 4, December 2024, Pages 709-722 PDF (581.55 K)
Document Type: Research Paper
DOI: 10.33899/berj.2024.185735
Authors
Shahad Faris Ibrahim^* ; Jahan Yahya AL-Hatem; Anwar Fakhre AL-Taee
University of Mosul / College of Education for Girls
Abstract
In this study, the molecular genetic index of the plant Epipremnum aureum was examined using the Specific-PCR technique. DNA fragments were amplified using the recommended Favor PrepTM (Genomic DNA Extraction Mini Kit) and amplified through Polymerase Chain Reaction. The molecular level detection of the Epipremnum aureum genome revealed that the sizes of the resulting bands matched the primer design (ITS1F: 5:TCCGTAGGTGAACCTGCGG-3) (ITS4R: 5:TCCTCCGCTTATTGATAGC-3), which are responsible for identifying the studied gene. The bands appeared during electrophoresis with a size of 473 base pairs. The nitrogen base sequences were entered into the NCBI-BLAST and MEGA 6.0 programs to determine their similarity to the existing and recorded genes in the Gene Bank. The results of the analysis showed a high similarity of 98.57 - 100% between the studied nucleotide sequences and the sequences recorded in the Gene Bank, with accession numbers (OP604476.1, PP297885.1). The genetic pattern tree indicated that they originated from countries far from Iraq and the regional area, namely the United States and South Korea. This study provides information for further research on molecular genetic relationships within the Araceae family, as well as molecular mutations and breeding of new varieties.
Keywords
PCR; DNA; molecular diagnostics

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