H. A. AL-Mayahi, S., K. Hassan, A., A. H. Alamery, S. (2025). ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS. , 23(1), 77-92. doi: 10.32649/ajas.2025.186566
S. H. A. AL-Mayahi; A. K. Hassan; S. A. H. Alamery. "ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS". , 23, 1, 2025, 77-92. doi: 10.32649/ajas.2025.186566
H. A. AL-Mayahi, S., K. Hassan, A., A. H. Alamery, S. (2025). 'ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS', , 23(1), pp. 77-92. doi: 10.32649/ajas.2025.186566
H. A. AL-Mayahi, S., K. Hassan, A., A. H. Alamery, S. ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS. , 2025; 23(1): 77-92. doi: 10.32649/ajas.2025.186566

ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS

Anbar Journal of Agricultural Sciences
Volume 23, Issue 1, June 2025, Pages 77-92    PDF (819.57 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2025.186566
Authors
S. H. A. AL-Mayahi1; A. K. Hassan2; S. A. H. Alamery1
1Dep Diagnostic, Plants Prot Office, Ministry Agric, Baghdad
2Dept. Plant Prot., Coll. of Agric. Engin. Sci., University of Baghdad
Abstract
This study was conducted to evaluate the efficiency of some PGPR isolates as biological agents in controling root rot disease (Rhizoctonia in broad beans) caused by the fungus Rhizoctonia solani. Tweenty six isolates were obtained through the Plant Growth Promoting Rhizobacteria sampling from different areas. Two isolates demonstrated a high antagonistic activity against the pathogenic fungus isolate. The identification of two isolates were Alcaligenes faecalis and Acinetobacter radioresistens was confirmed using vitek technology and nucleotide sequencing. The two strain sequences were deposited in the Gen Bank under accession numbers AR1 (OR101916) and AR2 (OR101917). The antagonistic activity was 100% for both isolates in the first method according to (Al-Mayahi and Hassan, 2021). While in the second method according to (Raspor at all, 2010), the two isolates of A. faecalis and A. radioresistens scored 58.92 and 57.14% inhibition at concentration 10-7 and 10-6, respectively. Secondary metabolites test of A. faecalis and A. radioresistens scored 93.33 and 85.55% inhibition rate at a concentration 10%.
Keywords
Broad bean; R. solani; PGPR; A. faecalis; A. radioresistens
Supplementary Files
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