H. Abdulmajeed, A., M. Muhammad, N. (2025). A MICROMORPHOLOGICAL STUDY OF SOME GENERA OF THE PAPILIONOIDEAE SUBFAMILY IN CENTRAL AND NORTHERN IRAQ. , 23(1), 216-234. doi: 10.32649/ajas.2025.186611
A. H. Abdulmajeed; N. M. Muhammad. "A MICROMORPHOLOGICAL STUDY OF SOME GENERA OF THE PAPILIONOIDEAE SUBFAMILY IN CENTRAL AND NORTHERN IRAQ". , 23, 1, 2025, 216-234. doi: 10.32649/ajas.2025.186611
H. Abdulmajeed, A., M. Muhammad, N. (2025). 'A MICROMORPHOLOGICAL STUDY OF SOME GENERA OF THE PAPILIONOIDEAE SUBFAMILY IN CENTRAL AND NORTHERN IRAQ', , 23(1), pp. 216-234. doi: 10.32649/ajas.2025.186611
H. Abdulmajeed, A., M. Muhammad, N. A MICROMORPHOLOGICAL STUDY OF SOME GENERA OF THE PAPILIONOIDEAE SUBFAMILY IN CENTRAL AND NORTHERN IRAQ. , 2025; 23(1): 216-234. doi: 10.32649/ajas.2025.186611

A MICROMORPHOLOGICAL STUDY OF SOME GENERA OF THE PAPILIONOIDEAE SUBFAMILY IN CENTRAL AND NORTHERN IRAQ

Anbar Journal of Agricultural Sciences
Volume 23, Issue 1, June 2025, Pages 216-234    PDF (2.03 M)
Document Type: Research Paper
DOI: 10.32649/ajas.2025.186611
Authors
A. H. Abdulmajeed1; N. M. Muhammad2
1Department of Horticulture and Landscaping, College of Agriculture, University of Anbar, Iraq
2College of Education for Pure Sciences, Tikrit University, Iraq
Abstract
This research involved a micro-morphological pollen study on 23 wild plant species belonging to six genera of the Papilionoideae subfamily growing in the central and northern districts of Iraq. These are Astragalus, Lathyrus, Medicago, Pisum, Trifolium, and Vicia. The samples were collected from February 2022 to June 2023. Altogether 34 field tours were made for conducting a comprehensive survey and to examine preserved herbarium specimen samples from the National Herbarium of Iraq and various universities. Pollen grains were examined for the arrangement of genera using a light microscope (LM 1000X) and a field-emission scanning electron microscope (FESEM). Diagnostic characteristics studied included the grain axis, apertures, E/P ratio, size, pollen grain shape, and surface ornamentation. The results show that the pollen grain were small-to-medium sized, tricolporate, isopolar, prolate, and spheroidal-to-subspheroidal. In addition, the surface ornamentation showed reticulate perforates, subperforate reticulate striates, reticulate sub-striate ornamentation, and reticulate perforate-striates. In conclusion, this study presents some morphological characteristics of pollen grains from the Papilionoideae subfamily in which LM (1000X) and FESEM were found to be useful and reliable tools in determining the importance of this classification in achieving historical knowledge of these seeds
Keywords
Papilionoideae; FESEM; Astragalus; Lathyrus; Micromorphological studies
References
  1. Aftab, R., and Perveen, A. (2006). A palynological study of some cultivated trees from Karachi. Pakistan journal of botany, 38(1): 15-28.
  2. Al-Saadi, M. (2013). A systematic study of the genus Trifolium Leguminosae in Iraq. Thesis. Coll. of Sci. Univ. Kufa.
  3. Aldobasissi, I. A. (2008). Morphological study of pollen grains in wild dicotyledons species growing in the Baghdad University Campus/ Jadriya. A Thesis, College of Science. University of Baghdad.
  4. Almosawi, M. B. H. (2024). Palynological study of the sleceted species in certain genera of the family Compositae (Asteraceae). SABRAO Journal of Breeding and Genetics, 56(2): 813-822. http://doi.org/10.54910/sabrao2024.56.2.32.
  5. Ayaz, S. (2023). Micrographic analysis of microspores of some members of the family Fabaceae, Lahore, Pakistan. Plantarum, 5(SI): 28.
  6. Bahadur, S., Long, W., Ahmad, M., Yaseen, M., Ullah, F., and Saqib, S. (2023). Exploration of pollen traits and their taxonomic relevance in selected taxa of the subfamily Papilionoideae from Hainan Island, China. Palynology, 47(2): 2144521. https://doi.org/10.1080/01916122.2022.2144521.
  7. Bano, A., Ahmad, M., Rashid, S., Zafar, M., Ashfaq, S., Rehman, S. U., ... and Ahmad, S. (2019). Microscopic investigations of some selected species of Papilionaceae through SEM and LM from Skardu valley, northern Pakistan. Microscopy research and technique, 82(4): 452-458. https://doi.org/10.1002/jemt.23188.
  8. Bano, A., Rashid, S., Ahmad, M., Bhatti, G. R., Yaseen, G., Anjum, F., ... and Kilic, O. (2020). Comparative pollen and foliar micromorphological studies using light microscopy and scanning electron microscopy of some selected species of Lamiaceae from Alpine Zone of Deosai Plateau, Western Himalayas. Microscopy Research and Technique, 83(6): 579-588. https://doi.org/10.1002/jemt.23448.
  9. Davis, P. H., and Heywood, V. H. (1963). Principles of angiosperm taxonomy. pp 556.
  10. De Almeida, L. T., de Azevedo Falcão, M. J., Mendonça, C. B. F., and Gonçalves-Esteves, V. (2024). Pollen diversity in Neotropical representatives of Dialioideae (Fabaceae). Flora, 315: 152508. https://doi.org/10.1016/j.flora.2024.152508.
  11. Erdtman, G. (1969). Handbook of Palynology Hafner. New York, NY.
  12. Erdtman, G. (1986). Pollen morphology and plant taxonomy: Angiosperms (an introduction to palynology). Brill.
  13. Ferguson, I. K. (1985). The role of pollen morphology in plant systematic. Asoc. Palinol. Leng. Esp, 2: 5-18.
  14. Gazer, H., Bous, E., and Mona, M. (2017). Palynotaxonomic studies on Boraginaceae in Saudi Arabia and its taxonomic significance. Life Science Journal, 14(4): 36-51. http://www.dx.doi.org/10.7537/marslsj140417.06.
  15. Gonçalves-Esteves, V., Mezzonato-Pires, A. C., Marinho, E. B., de Souza, R. M. B. S., Esteves, R. L., Cartaxo-Pinto, S., and Mendonça, C. B. F. (2022). The importance of palynology to taxonomy. In aspects of Brazilian floristic diversity: From botany to traditional communities. Chapter 31, 10th, Cham, Springer, pp. 119-134. https://doi.org/10.1007/978-3-031-07453-0_5.
  16. Guest, E., and Townsend, C. C. (1966). Flora of Iraq: Leguminales (Vol. 3). Ministry of Agriculture of the Republic of Iraq.
  17. Gul, F., Malik, K., Qureshi, R., Ahmad, M., Ansari, L., Zafar, M., ... and Rashid, N. (2022). Palyno‐morphological attributes of some selected plant species of family Asteraceae from district Dera Ismail Khan, KPK, Pakistan. Microscopy Research and Technique, 85(4): 1392-1409. https://doi.org/10.1002/jemt.24003.
  18. Gunes, F., and Aytug, B. (2010). Pollen morphology of the genus Lathyrus (Fabaceae) section Pratensis in Turkey. Int. J. Agric. Biol, 12(1): 96-100.
  19. Günes, F., and Cirpici, A. (1998). Pollen morphology of some Lathyrus species (L. undulatus Boiss., L. sylvestris L., L. ochrus (L.) DC) of Istanbul area (In Tukish). In Proceedings of the Symposium on Quercus vulcanica and Flora of Turkey, Cantay Kitapevi, Laleli-Istanbul, 431-440.
  20. Güneş, F. A. T. M. A. (2012). Seed morphology and their systematic importance of Lathyrus taxa belonging to Platystylis (= Lathyrostylis) section (Fabaceae) from Turkey. African Journal of Agricultural Research, 7(2): 265-277. DOI: 10.5897/AJAR11.1502.
  21. Hasson, S. M., AL-Qaraawi, H. A. H., and Hashim, R. (2009). Taxonomic Study of the Genus Phlomis L. (Lamiaceae) in Iraq Based on Trichome and Palynological Characters. Journal of Global Pharma Technology, 12(06):151-158.
  22. Kamel, M. A., El Hadidy, A. M., Hamed, S. T., and Hussein, N. R. (2019). A Palynological review for some species of family Boraginaceae Juss. From the Egyptian Flora. Annual Research and Review in Biology, 30(3): 1-16. DOI: 10.9734/ARRB/2018/46408.
  23. Kayani, S., Hussain, M., Ahmad, M., Zafar, M., Sultana, S., Butt, M. A., ... and Mir, S. (2019). Scanning electron microscopy (SEM) and light microscopy (LM)‐based Palyno‐morphological views of Solanaceae in Western Himalaya. Microscopy Research and Technique, 82(2): 63-74. https://doi.org/10.1002/jemt.23097.
  24. Khal, L. H., Tahir, N. A. R., and Abdul-Razaq, R. T. (2023). Molecular variation in some taxa of genus Astragalus L. (Fabaceae) in the Iraqi Kurdistan Region. Horticulturae, 9(10): 1110. https://doi.org/10.3390/horticulturae9101110.
  25. Khal, L. H. (2024). Systematic study in some taxa of the genus Astragalus (Fabaceae) in districts of Kurdistan-Iraq. (Doctoral dissertation, Faculty of Agricultural Sciences, University of Sulaimani).
  26. Khan, A., Ahmad, M., Sultan, A., Ullah, Z., Majeed, K., Ullah, T., and Zafar, M. (2024). Contribution to the pollen morphology of Astragalus L. section Aegacantha Bunge (Galegeae-Fabaceae) and its systematic significance. Palynology, 48(1): 2264352. https://doi.org/10.1080/01916122.2023.2264352.
  27. Khan, A. S., Ahmad, M., Zafar, M., Athar, M., Ozdemir, F. A., Gilani, S. A. A., ... and Khan, S. U. (2020). Morphological characterization of Hypnaceae (Bryopsida, Hypnales): Investigating four genera from Western Himalayas by using LM and SEM techniques. Microscopy Research and Technique, 83(6): 676-690. https://doi.org/10.1002/jemt.23458.
  28. Khan, I., Akhtar, N., and Khan, S. A. (2020). Palynological investigation of some selected species of family Fabaceae from Pakistan, using light and scanning electron microscopy techniques. Microscopy research and technique, 83(3): 223-231. https://doi.org/10.1002/jemt.23405.
  29. Khan, S., Jan, G., Ahmad, M., Gul, F., Zafar, M., Mangi, J. U. D., Bibi, H., Sultana, S., Usma, A. and Majeed, S. (2021). Morpho‐palynological assessment of some species of family Asteraceae and Lamiaceae of District Bannu, Pakistan on the bases of light microscope and scanning electron microscopy. Microscopy Research and Technique. 84(6):31220-1232. doi.org/10.1002/jemt.23681
  30. Nazish, M., Bibi, F., Alwahibi, M. S., Elshikh, M. S., and Zaman, W. (2024). Exploration of salinity effect on palyno-morphological characteristics of plant species collected from salt range. Bangladesh Journal of Plant Taxonomy, 31(1): 33-49. https://doi.org/10.3329/bjpt.v31i1.74385.
  31. Perveen, A., and Qaiser, M. (1998). Pollen Flora of Pakistan-VIII Leguminosae (subfamily: Papilionoideae). Turkish Journal of Botany, 22(2): 73-92.
  32. Pritha Bhattacharya, P. B., Sayani Biswas, S. B., and Pal, J. K. (2015). Palyno-taxonomic study of some plant taxa of Fabaceae from Arambagh region of Hooghly district, West Bengal, eastern India, 6(1): 27-34.
  33. Radford, A. E., Dikson, W. C., Massey, J. R., and. Bell, C. R. (1974). Vascular plant systematic. Harper and Row. 891.
  34. Raza, J., Ahmad, M., Zafar, M., Athar, M., Sultana, S., Majeed, S., ... and Hussain, A. (2020). Comparative foliar anatomical and pollen morphological studies of Acanthaceae using light microscope and scanning electron microscope for effective microteaching in community. Microscopy Research and Technique, 83(9): 1103-1117. https://doi.org/10.1002/jemt.23502.
  35. Rukhshinda Aftab, R. A., and Perveen, A. (2006). A palynological study of some cultivated trees from Karachi. Pakistan journal of botany, (1): 15-38.
  36. Saas, J. E. (1998). Botanical Microtechnique. 3rd ed. The Low State University Press, 228.
  37. Ullah, I., Ahmad, M., Jabeen, A., Yusuf, M. O., Arfan, M., Kilic, O., ... and Usma, A. (2021). Palyno‐morphological characterization of selected allergenic taxa of family Poaceae from Islamabad‐Pakistan using microscopic techniques. Microscopy Research and Technique, 84(11): 2544-2558. https://doi.org/10.1002/jemt.23803.
  38. Wilson, L. R., and Goodman, G. J. (1963). Techniques of palynology–Part I. Collection and preparation of modern spores and pollen. Oklahoma Geology Notes, 23: 167-171.
  39. Xiao, J., Shang, Z., Xu, J., Jia, X., and Xiao, S. (2023). The neolithic culture and paleogeographic environment evolution in the eastern Jianghuai area. Land, 12(1): 156. https://doi.org/10.3390/land12010156.
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