Conventional and molecular identification of Giardia intestinalis in human stool samples in Baghdad Province, Iraq.

Abdulsada, Amal Kamel; Alfatlawi, Monyer AA

doi:10.29079/qjvms.2024.151348.1025

	Conventional and molecular identification of Giardia intestinalis in human stool samples in Baghdad Province, Iraq.
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Issue 1, January 2025, Pages 14-19 PDF (441.69 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2024.151348.1025
Authors
Amal Kamel Abdulsada¹; Monyer AA Alfatlawi^* ²
¹College of Veterinary Medicine University of Al-Qadisiyah Iraq
²Department of Veterinary Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.
Abstract
Human diarrhea is caused by the zoonotic protozoan Giardia lamblia. This parasite is very important, so it might serve as a study object. The purpose of the work described below was to identify G. lamblia in human stool samples from Baghdad province, Iraq. 140 fecal samples constituted an experiment. The samples were collected throughout the year. PCR, partial gene sequencing, and microscopic analysis were performed. The microscopic approach identified cysts at the highest rate (14/59, 23.7%) in the month of January, and PCR was able to determine that the percentage of humans certified as sick was 21/21 (99.09%). The phylogenetic tree shows that the strains of isolates are very close to the world strains from Poland and Iran. Overall, the work at hand provides substantial pieces of information on the occurrence of G. lamblia in human stool.
Keywords
Giardia; human diarrhea; protozoa

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