Abed, A., Alfatlawi, M. (2025). Haemonchus contortus: Conventional and molecular identification and characterization via sequencing-based phylogenetic analysis in sheep. , 24(1), 20-25. doi: 10.29079/qjvms.2024.151711.1029
Athraa Najim Abed; Monyer AA Alfatlawi. "Haemonchus contortus: Conventional and molecular identification and characterization via sequencing-based phylogenetic analysis in sheep". , 24, 1, 2025, 20-25. doi: 10.29079/qjvms.2024.151711.1029
Abed, A., Alfatlawi, M. (2025). 'Haemonchus contortus: Conventional and molecular identification and characterization via sequencing-based phylogenetic analysis in sheep', , 24(1), pp. 20-25. doi: 10.29079/qjvms.2024.151711.1029
Abed, A., Alfatlawi, M. Haemonchus contortus: Conventional and molecular identification and characterization via sequencing-based phylogenetic analysis in sheep. , 2025; 24(1): 20-25. doi: 10.29079/qjvms.2024.151711.1029

Haemonchus contortus: Conventional and molecular identification and characterization via sequencing-based phylogenetic analysis in sheep

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Issue 1, January 2025, Pages 20-25    PDF (517.94 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2024.151711.1029
Authors
Athraa Najim Abed; Monyer AA Alfatlawi*
Department of Veterinary Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.
Abstract
The purpose of this study, conducted from 01-October-2023 to 28-February-2024, was to determine the prevalence and genetic diversity of Haemonchus contortus Infection from sheep in Al-Diwaniyah slaughterhouse, it was carried out to investigate the level of infection at two levels stages; adult's animal, and human, and also helped us to evaluate the genetic similarities between local isolates from Iraq in comparison with other strains of H. contortus from all over the world. In the slaughterhouse in Al-Diwaniyah City, 250 carcasses were sampled. The samples were divided into two tubes, one of them used for microscopic examination by lacto phenol-solution and the other used for molecular tests by using 70% ethanol. Targeting the β-tubulin region by PCR was done, which was followed with the positive PCR-based sequencing. The findings of this study revealed high prevalence of H. contortus infection rate in sheep which leads to parasite transmission among the animals. In addition, a high level of genetic similarities was seen between local isolates from Iraq and strains from Sweden, Canada, Switzerland, the USA, and the Netherland. Overall, this study concludes that the level of infections by H. contortus. High genetic similarities, with world strains, indicating high diversity and common ancestor.
Keywords
Enteral parasites; Haemonchus contortus; roundworms
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