khalaf, J., Khadhim, H. (2025). Isolation and identification of adipose tissue derived-derived mesenchymal stem cells from cats. , 24(1), 26-30. doi: 10.29079/qjvms.2024.152375.1031
jassim mohammed khalaf; Hameed Ali Khadhim. "Isolation and identification of adipose tissue derived-derived mesenchymal stem cells from cats". , 24, 1, 2025, 26-30. doi: 10.29079/qjvms.2024.152375.1031
khalaf, J., Khadhim, H. (2025). 'Isolation and identification of adipose tissue derived-derived mesenchymal stem cells from cats', , 24(1), pp. 26-30. doi: 10.29079/qjvms.2024.152375.1031
khalaf, J., Khadhim, H. Isolation and identification of adipose tissue derived-derived mesenchymal stem cells from cats. , 2025; 24(1): 26-30. doi: 10.29079/qjvms.2024.152375.1031

Isolation and identification of adipose tissue derived-derived mesenchymal stem cells from cats

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Issue 1, January 2025, Pages 26-30    PDF (340.67 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2024.152375.1031
Authors
jassim mohammed khalaf* 1; Hameed Ali Khadhim2
1Department of Veterinary Surgery and Obstetrics, College of Veterinary Medicine, University of Kerbala, Karbala, Iraq
2Department of veterinary Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
Abstract
Mesenchymal stem cells derived from adipose tissue (AD-MSCs) have shown promise in the field of regenerative medicine. This work aimed to evaluate the discovery of pre-committed mesenchymal lineages in cat adipose-derived stem cells that create multipotent stromal cells in response to inductive extracellular signals. A cat's inguinal area yielded three grams of fat subcutaneous tissue, which was carefully extracted to produce mesenchymal stem cells (MSCs) that would be enlarged by a fourth passage. Active mesenchymal stem cell proliferation was seen in the fourth passage. Additionally, the study showed that cat MSCs had the innate capacity to stimulate AD-MSC identification using immunocytochemistry examination. Cats' AD-MSCs were consistently positive for CD90 and CD44 at passage three, according to immunophenotypic analysis. Nevertheless, it was consistently discovered that MSCs lacked the hematopoietic specific markers CD34 and CD45.In conclusion, mesenchymal stem cells may be extracted from cat adipose tissue using the direct plating technique, which works well. These cells are an important source of stem cells that might be used for the replacement of organs.
Keywords
Adipose tissue; cat; immunocytochemistry; stem cells
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