Kadhim, E., A'aiz, N. (2025). Morphological and molecular identification of the larvae causing myiasis in cattle. , 24(1), 73-78. doi: 10.29079/qjvms.2024.155207.1048
Esraa Ayad Kadhim; Noaman Naji A'aiz. "Morphological and molecular identification of the larvae causing myiasis in cattle". , 24, 1, 2025, 73-78. doi: 10.29079/qjvms.2024.155207.1048
Kadhim, E., A'aiz, N. (2025). 'Morphological and molecular identification of the larvae causing myiasis in cattle', , 24(1), pp. 73-78. doi: 10.29079/qjvms.2024.155207.1048
Kadhim, E., A'aiz, N. Morphological and molecular identification of the larvae causing myiasis in cattle. , 2025; 24(1): 73-78. doi: 10.29079/qjvms.2024.155207.1048

Morphological and molecular identification of the larvae causing myiasis in cattle

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Issue 1, January 2025, Pages 73-78    PDF (469.9 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2024.155207.1048
Authors
Esraa Ayad Kadhim* ; Noaman Naji A'aiz
Department of Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq
Abstract
A myiasis infestation occurs when dipterous larvae infest living vertebrates, includeing the humans and animals. Depending on where they live and how they interact with their host, dipterous larvae in mammals can feed on either living tissues or dead tissue, bodily fluids, or food that has been consumed. They can also cause a variety of infestations. The Caliphoridae family is considered the most important family causing myiasis, so the research's goal was to identify the species causing infestation in cows. Methods: Infested animals (50 cattle) were examined and larvae  from each one were collected from each animal, then the species firstly studied and identified microscopically  based on their the morphological and anatomical characteristics of larvae followed by study the effect of certain risk factors related to the infestation occur, then they were examined using PCR technique through amplifying the small subunit ribosomal RNA of chrysomya pseudolucilia (CPS) gene to confirm the diagnosis, also their genetic sequence and phylogenetic tree were analysis. Results: The results showed that all larval samples isolated from infected cows represented Chrysooma bezziana according to the morphological examination of these larvae. PCR examination and genetic sequence analysis of some samples (10) confirmed the species of fly diagnosed microscopically, and it was noted through studying the genetic tree of these samples that there is a similarity (95-97%) with one of the global isolates. Regarding the risk factors studied, it was noted that there is an effect of the age and sex of the animal on the infestation rate, and it was noted that its rates were affected by the site of infection on the body and the geographical location of the animal, and no significant effect was noted for the year months. Conclusion: From the results obtained in this study, we conclude that most cases of worms found Chrysoma bezziana causes cows, and it seems that it is still endemic in Iraq / Diwaniyah despite the therapeutic attempts, and that the local isolates are of external origin due to the great similarity with the global isolates.
Keywords
Calliphoridae; cattle; Chrysoma bezziana; PCR
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