Bari, N., Salman, A. (2025). Trends in Aflatoxin B1 Contamination in Broiler Feed: A Three-Year Study from Duhok Governorate. , 24(1), 68-72. doi: 10.29079/qjvms.2025.154037.1044
Niwar Shamsaddeen Bari; Araz O. Salman. "Trends in Aflatoxin B1 Contamination in Broiler Feed: A Three-Year Study from Duhok Governorate". , 24, 1, 2025, 68-72. doi: 10.29079/qjvms.2025.154037.1044
Bari, N., Salman, A. (2025). 'Trends in Aflatoxin B1 Contamination in Broiler Feed: A Three-Year Study from Duhok Governorate', , 24(1), pp. 68-72. doi: 10.29079/qjvms.2025.154037.1044
Bari, N., Salman, A. Trends in Aflatoxin B1 Contamination in Broiler Feed: A Three-Year Study from Duhok Governorate. , 2025; 24(1): 68-72. doi: 10.29079/qjvms.2025.154037.1044

Trends in Aflatoxin B1 Contamination in Broiler Feed: A Three-Year Study from Duhok Governorate

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Issue 1, January 2025, Pages 68-72    PDF (344.83 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2025.154037.1044
Authors
Niwar Shamsaddeen Bari* ; Araz O. Salman
Department of Pathology and Microbiology, College of Veterinary Medicine, University of Duhok, Duhok, Iraq
Abstract
This study assesses the levels of aflatoxin B1 contamination in broiler feed samples that were taken from farms in the Duhok Governorate between January 2020 and August 2022. An ELISA technique used to evaluate 213 feed samples in total. The data indicated a significant increase in aflatoxin B1 contamination, with 22 samples (10.32%) testing negative and 191 samples (89.67%) testing positive. Every year from 2020 to 2022, the proportion of samples that tested positive for contamination increased, going from 76.36% in 2020 to 91.50% in 2021 and 100% in 2022. The concentration of aflatoxin B1 also showed an increased trend, with mean values of 5.351 ppb in 2020 and 6.338 ppb in 2022. Descriptive statistics indicated considerable variability in aflatoxin levels, with the highest mean observed in 2022. ANOVA results confirmed statistically significant differences between years (F-value = 4.36, p-value = 0.014), highlighting a concerning escalation in contamination levels. This rise emphasizes the need for improved management and monitoring techniques to reduce aflatoxin exposure. The results are consistent with prior research showing elevated levels of contamination brought on by environmental factors and feed management techniques. The research highlights the significance of enforcing more stringent quality control protocols to guarantee feed safety and safeguard the well-being of chickens.
Keywords
Aflatoxin; Broiler; ELISA; Feed
References
  1. Liu Y, Wu F. Global burden of aflatoxin-induced diseases. Econ Aff. 2010;30(2):193-207. https://doi.org/10.1289/ehp.0901388
  2. Pitt JI, Hocking AD. Fungi and food spoilage. Springer; 2009. https://doi.org/10.1007/978-0-387-92207-2
  3. Benkerroum N. Aflatoxins: Producing-molds, structure, health issues and incidence in Southeast Asian and Sub-Saharan African countries. Int J Environ Res Public Health. 2020;17(4):1215. https://doi.org/10.3390/ijerph17041215
  4. Li C, Liu X, Wu J, Ji X, Xu Q. Research progress in toxicological effects and mechanism of aflatoxin B1 toxin. PeerJ. 2022;10:e13850. https://doi.org/10.7717/peerj.13850
  5. Yunus AW, Razzazi-Fazeli E, Bohm J. Aflatoxin B1 affecting broiler performance, immunity, and gastrointestinal tract: A review of history and contemporary issues. Toxins. 2011;3(6):566-90. https://doi.org/10.3390/toxins3060566
  6. Kajuna FF, Temba BA, Mosha RD. Surveillance of aflatoxin B1 contamination in chicken commercial feeds in Morogoro, Tanzania. Livest Res Rural Dev. 2013;25(3):51. https://doi.org/10.4314/thrb.v12i4.51792
  7. Munkvold GP. Cultural and genetic approaches to managing mycotoxins in maize. Annu Rev Phytopathol. 2003;41(1):99-116. https://doi.org/10.1146/annurev.phyto.41.052002.095510
  8. Motbaynor A, Kassaye D, Keffale M, Wasihun P. Magnitude of aflatoxigenic Aspergillus species, level of aflatoxin B1, and associated factors in stored feed at poultry farms in Dire Dawa, Ethiopia. Vet Med Int. 2021;2021:6638083. https://doi.org/10.1155/2021/6638083
  9. Awuchi CG, Amagwula IO, Priya P, Kumar R, Yezdani U, Khan MG. Aflatoxins in foods and feeds: A review on health implications, detection, and control. Bull Environ Pharmacol Life Sci. 2020;9:149-55.
  10. Hussein HS, Brasel JM. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology. 2001;167(2):101-34. https://doi.org/10.1016/s0300-483x(01)00471-1
  11. Guengerich FP, Johnson WW, Shimada T, Ueng YF, Yamazaki H, Langouët S. Activation and detoxication of aflatoxin B1. Mutat Res. 1998;402(1-2):121-8. https://doi.org/10.1016/s0027-5107(97)00289-3
  12. Diaz GJ, Murcia HW. Biotransformation of aflatoxin B1 and its relationship with the differential toxicological response to aflatoxin in commercial poultry species. Aflatoxins-Biochem Mol Biol. 2011;1:3-20. https://doi.org/10.5772/22109
  13. Ferreira RG, Cardoso MV, De Souza Furtado KM, Espíndola KMM, Amorim RP, Monteiro MC. Epigenetic alterations caused by aflatoxin B1: A public health risk in the induction of hepatocellular carcinoma. Transl Res. 2019;204:51-71. https://doi.org/10.1016/j.trsl.2018.09.001
  14. Cao W, Yu P, Yang K, Cao D. Aflatoxin B1: Metabolism, toxicology, and its involvement in oxidative stress and cancer development. Toxicol Mech Methods. 2022;32(6):395-419. https://doi.org/10.1080/15376516.2021.2021339
  15. Wang Y, Wang X, Li Q. Aflatoxin B1 in poultry liver: Toxic mechanism. Toxicon. 2023;107262. https://doi.org/10.1016/j.toxicon.2023.107262
  16. Monson MS, Coulombe RA, Reed KM. Aflatoxicosis: Lessons from toxicity and responses to aflatoxin B1 in poultry. Agric. 2015;5(3):742-77. https://doi.org/10.3390/agriculture5030742
  17. Mishra HN, Das M. A review of aflatoxin contamination and its management in crops. Food Rev Int. 2003;19(2):245-72. https://doi.org/10.1080/10408690390826518
  18. Joint FAO/WHO Expert Committee on Food Additives. Safety evaluation of certain mycotoxins in food. Food Agric Org. 2001;56. https://doi.org/10.1016/s0168-1605(02)00160-5
  19. Mushtaq S, Daş YK, Aksoy A. Analytical methods for determination of aflatoxin B1 in animal feeds and feedstuffs. Erciyes Univ Vet Fak Derg. 2020;17(2):173-9. https://doi.org/10.32707/ercivet.760828
  20. Zhang N, Han X, Zhao Y, Li Y, Meng J, Zhang H, Liang J. Removal of aflatoxin B1 and zearalenone by clay mineral materials: In the animal industry and environment. Appl Clay Sci. 2022;228:106614. https://doi.org/10.1016/j.clay.2022.106614
  21. Negash D. A review of aflatoxin: occurrence, prevention, and gaps in both food and feed safety. J Appl Microbiol Res. 2018;1(1):35-43. https://doi.org/10.15406/jnhfe.2018.08.00268
  22. Sipos P, Peles F, Brassó DL, Béri B, Pusztahelyi T, Pócsi I, Győri Z. Physical and chemical methods for reduction in aflatoxin content of feed and food. Toxins. 2021;13(3):204. https://doi.org/10.3390/toxins13030204
  23. Lubna MA, Debnath M, Hossaini F. Detection of aflatoxin in poultry feed and feed materials through immuno-based assay from different poultry farms and feed factories in Bangladesh. Bangladesh J Microbiol. 2018;35(1):75-8. https://doi.org/10.3329/bjm.v35i1.39807
  24. Bhat R, Rai RV, Karim AA. Mycotoxins in food and feed: present status and future concerns. Compr Rev Food Sci Food Saf. 2010;9(1):57-81. https://doi.org/10.1111/j.1541-4337.2009.00094.x
  25. Villers P. Aflatoxins and safe storage. Front Microbiol. 2014;5:158. https://doi.org/10.3389/fmicb.2014.00158
  26. Naveed M, Haleem KS, Ghazanfar S, Tauseef I, Bano N, Adetunji CO, et al. Quantitative estimation of aflatoxin level in poultry feed in selected poultry farms. Biomed Res Int. 2022;2022:5397561. https://doi.org/10.1155/2022/5397561
  27. Rossi CN, Takabayashi CR, Ono MA, Saito GH, Itano EN, Kawamura O, et al. Immunoassay based on monoclonal antibody for aflatoxin detection in poultry feed. Food Chem. 2012;132(4):2211-6. https://doi.org/10.1016/j.foodchem.2011.12.067
  28. Shephard GS. Risk assessment of aflatoxins in food and feed: A review. Food Addit Contam Part A. 2008;25(5):277-93. https://doi.org/10.1080/02652030802036222
  29. Alshawabkeh K, Alkhalaileh NI, Abdelqader A, Al-Fataftah ARA, Herzallah SM. Occurrence of aflatoxin B1 in poultry feed and feed ingredients in Jordan using ELISA and HPLC. Am-Eurasian J Toxicol Sci. 2015;7(4):316-20.
  30. Kumar N, Sheekha RM, Meena H. Toxigenic fungi: Their impact and management. Insect Sci Exp. 2020;160.
  31. Smith JE. Aflatoxins. In: Handbook of plant and fungal toxicants. CRC Press; 2020. p. 269-85. https://doi.org/10.1201/9780429281952-19
  32. Coppock RW, Christian RG, Jacobsen BJ. Aflatoxins. In: Veterinary toxicology. Academic Press; 2018. p. 983-94. https://doi.org/10.1016/b978-0-12-811410-0.00069-6
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