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Molecular detection of Methicillin-Resistant Staphylococcus aureus isolated from Subclinical Mastitis Cows in Basrah Province. | ||
| Basrah Journal of Veterinary Research | ||
| Volume 24, Issue 1, March 2025, Pages 131-141 PDF (899.64 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvr.2025.155550.1166 | ||
| Author | ||
| Zainab Abdulameer Farhan, Nawres Norri Jaber, Rana Adnan Fayez. | ||
| Abstract | ||
| Out of 200 milk samples collected from apparently normal cows in Basrah province from 21 November 2023 to 6 February 2024, 95 (48)% were positive for the CMT test. This study used various techniques to detect the presence of staphylococcus aureus, including conventional microbiological assays (mannitol salts agar and CHROMagar™ Staph aureus) and molecular methods (amplifying the nuc gene using polymerase chain reaction ). The percentage of S. aureus isolates was 38 (19 % ). From 38 ( 19 %) S. aureus isolates, 27 (71%) carried the mecA gene, 6 (16%) carried pvl, while none of the isolates carried the mecC gene. | ||
| Keywords | ||
| Mastitis; Subclinical mastitis; Cows; MRSA | ||
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