- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Classification of Some Cultivars Species of The Genus Bougainvillea Comm. ex Juss. Cultivated In Northern Iraq Using RAPD Markers | ||
| Journal of Education and Science | ||
| Volume 34, Issue 3, July 2025, Pages 16-25 PDF (897.43 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/edusj.2025.157287.1534 | ||
| Authors | ||
| Raad Hamad Al-Badany* 1; Aamer Al-Mathidy2 | ||
| 1Nineveh Education Directorate, Mosul, Iraq | ||
| 2Department of Biology, College of Education for Pure Science, University of Mosul, Mosul, Iraq | ||
| Abstract | ||
| The research dealt with a molecular taxonomic study of 6 cultivars of species of the genus Bougainvillea Comm. ex Juss., which included the cultivars ʽMary palmerʼ, ʽRasberry iceʼ, for the species B. spectabilis Willd, ʽRoyal purpleʼ, ʽFormosaʼ for the species B. glabra Choisy, ʽThimmaʼ for the species B. peruviana Hum. And Bon., and ʽCalifornia goldʼ for the species B. x buttiana using eight different random primers for PCR, which was used to multiply and amplify the DNA fragments using random amplified polymorphic DNA (RAPD) markers. Through sample migration on an agarose gel, DNA was extracted from the leaves of cultivars and genetic diversity between amplified pieces for each cultivar was found. The findings indicated that differences in the locations on the genomes of the studied cultivars and the production of different bands revealed on the agarose gel, which reflects the magnitude of genetic variation depending on the primer used. As the primers produced (309) total bands resulting from (95) binding sites, including (12) monomorphic binding sites and (83) polymorphic binding sites distinct for the studied cultivars. The results of the cluster analysis (dendrogram) for the cultivars species of the genus Bougainvillea which depend on the values of the similarity showed the presence of clear variations among the studied cultivars. The study concluded that the RAPD-PCR technique used in molecular classification based on molecular markers is a powerful tool for identifying and classifying cultivars of species of the genus Bougainvillea Comm. ex Juss. and detecting genetic relationships among them. | ||
| Keywords | ||
| dendrogram; PCR; primer | ||
|
Supplementary Files
|
||
|
Statistics Article View: 112 PDF Download: 128 |
||
