I. Al-Jugifi, W., H. Nafea, H., N. Alhayani, N. (2025). IMPACT OF SUPPLEMENTING DIETS WITH CHITOSAN AND PROBIOTICS ON CERTAIN BLOOD PROPERTIES OF BROILERS. , 23(1), 656-666. doi: 10.32649/ajas.2025.187598
W. I. Al-Jugifi; H. H. Nafea; N. N. Alhayani. "IMPACT OF SUPPLEMENTING DIETS WITH CHITOSAN AND PROBIOTICS ON CERTAIN BLOOD PROPERTIES OF BROILERS". , 23, 1, 2025, 656-666. doi: 10.32649/ajas.2025.187598
I. Al-Jugifi, W., H. Nafea, H., N. Alhayani, N. (2025). 'IMPACT OF SUPPLEMENTING DIETS WITH CHITOSAN AND PROBIOTICS ON CERTAIN BLOOD PROPERTIES OF BROILERS', , 23(1), pp. 656-666. doi: 10.32649/ajas.2025.187598
I. Al-Jugifi, W., H. Nafea, H., N. Alhayani, N. IMPACT OF SUPPLEMENTING DIETS WITH CHITOSAN AND PROBIOTICS ON CERTAIN BLOOD PROPERTIES OF BROILERS. , 2025; 23(1): 656-666. doi: 10.32649/ajas.2025.187598

IMPACT OF SUPPLEMENTING DIETS WITH CHITOSAN AND PROBIOTICS ON CERTAIN BLOOD PROPERTIES OF BROILERS

Anbar Journal of Agricultural Sciences
Volume 23, Issue 1, June 2025, Pages 656-666    PDF (462.96 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2025.187598
Authors
W. I. Al-Jugifi1; H. H. Nafea1; N. N. Alhayani2
1Department of Animal Production, Faculty of Agriculture, University of Anbar, Iraq
2College of Medicine, University of Anbar
Abstract
This experiment was conducted at the poultry farm of the Animal Production Department, College of Agriculture, University of Anbar from 28 December 2023 to 8 February 2024 (42 days). It aimed to compare the effect of adding two different levels of chitosan and probiotics to the diet and their combinations in the physiological performance and some blood characteristics of broilers from the age of 6 weeks. The study used 10 one-day-old Ross chicks of 40 g average weight. The chicks were distributed into 7 treatments of 3 replicates each (10 chicks for each replicate). The first treatment (T1) was the control (without any addition), the T2 and T3 treatments included adding chitosan at 1 and 2 g/kg feed, respectively. The T4 and T5 treatments included adding probiotic at 1 and 2 g/kg of feed, respectively. The T6 treatment included the addition of chitosan and probiotics at 0.5 g/kg of feed each while T7 included the addition of chitosan and probiotics at 1 g/kg feed each. Results show that the T5 treatment had significant superiority (P≤0.05) in the PCV and hemoglobin values compared to T1, T2, and T4, and did not differ significantly from the other treatments. Treatment T5 also continued to be superior in the number of red blood cells compared to T1, T2, T3, and T4 and did not differ significantly from the other treatments. There was also a significant decrease (P≤0.05) in the concentration of blood plasma glucose in T2 compared to T6, while treatment T2 recorded a significant increase in total protein concentration over T3, T4, and T6. Also, treatment T2 showed a significant increase in globulin concentration compared to T3 and T4 but was not significantly different from the other treatments. Lipid profiles recorded a significant decrease (P≤0.05) in the concentration of blood plasma cholesterol and low-density lipoproteins in treatment T6 compared to T2 and T4, but were not significantly different from the other treatments. Liver enzymes recorded a significant decrease (P≤0.05) in the AST enzyme level in treatment T2 over T4 and T5, but was not significantly at variance from the other treatments
Keywords
Chitosan; Probiotics; Blood characteristics; Broilers
References
  1. Abdel-Rahman, M. A., et al. (2020). Effects of probiotics supplementation on growth performance, feed utilization, and gut health of broiler chickens. Poultry Science, 99(3): 1445-1455.
  2. Adil, S., Aldhalmi, A. K., Wani, M. A., Baba, I. A., Sheikh, I. U., Abd El-Hack, M. E., ... and Abuljadayel, D. A. (2024). Impacts of dietary supplementation of chitosan nanoparticles on growth, carcass traits nutrient digestibility, blood biochemistry, intestinal microbial load, and meat quality of broilers. Translational Animal Science, 8: txae134.‏ https://doi.org/10.1093/tas/txae134.
  3. AFM, A.-E., Saed, Z. J. M., Naser, A. S., Mohammed, Th. T., Abdulateef, S. M., ALKhalani, F. M. H., & Abdulateef, F. (2020). The Role of Adding Sodium Chloride in Broiler Chicks Diets to Improve Production Performance and Antioxidant Status during Heat Stress. Annals of Tropical Medicine and Public Health, 23(16). https://doi.org/10.36295/asro.2020.231612.
  4. Alkhalf, A., Alhaj, M., and Al-Homidan, I. (2010). Influence of probiotic supplementation on blood parameters and growth performance in broiler chickens. Saudi journal of biological sciences, 17(3): 219-225.‏ https://doi.org/10.1016/j.sjbs.2010.04.005.
  5. Al-Marzooqi, W., El-Zaiat, H. M., El Tahir, Y., Al-Kharousi, K., and Hassan, S. K. (2024). Influence of chitosan dietary supplement on growth performance, blood indices, and characteristics of meat quality in chickens. J. Vet. Res, 4(3): 49-60.‏ https://doi.org/10.51585/gjvr.2024.3.0097.
  6. Al-Yaseen, A. A., and Abdel-Abbas, M. H. (2010). Ministry of Higher Education and Scientific Poultry Feed Research, University of Baghdad/College of Agriculture.
  7. Arif, M., Akteruzzaman, M., Islam, S. S., Das, B. C., Siddique, M. P., and Kabir, S. L. (2021). Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickens. Veterinary and Animal Science, 14: 100216. https://doi.org/10.1016/j.vas.2021.100216.
  8. Chen, X., Zhang, Y., Ma, W., Zhu, Y., Wu, X., and Wang, Z. (2020). Effects of Cordyceps militaris polysaccharide on egg production, egg quality and Caecal microbiota of layer hens. Journal of World's Poultry Research, 10(1): 41-51.
  9. Chen, Y., et al. (2019). Effects of dietary probiotics on immune response and intestinal health in broilers. Frontiers in Veterinary Science, 6: 108.
  10. Ciurescu, G., Dumitru, M., Gheorghe, A., Untea, A. E., and Drăghici, R. (2020). Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources. Poultry science, 99(11): 5960-5971. https://doi.org/10.1016/j.psj.2020.08.075.
  11. Duncan, D. B. (1955). Multiple range and multiple F tests. biometrics, 11(1): 1-42. https://doi.org/10.2307/3001478.
  12. Elnesr, S. S., Elwan, H. A., El Sabry, M. I., Shehata, A. M., and Alagawany, M. (2022). Impact of chitosan on productive and physiological performance and gut health of poultry. World's Poultry Science Journal, 78(2): 483-498.‏ https://doi.org/10.1080/00439339.2022.2041992.
  13. Gao, P., et al. (2017). Probiotics improve intestinal microbiota and immunity in broilers. Animal Feed Science and Technology, 234: 142-152.
  14. Halder, N., Sunder, J., De, A. K., Bhattacharya, D., and Joardar, S. N. (2024). Probiotics in poultry: a comprehensive review. The Journal of Basic and Applied Zoology, 85(1): 23.‏ https://doi.org/10.1186/s41936-024-00379-5.
  15. Hazaa, A. A., and Nafe, H. H. (2024). Response of broilers to dimethylglycine addition to normal and low-energy diet on physiological performance and antioxidant status. Anbar Journal of Agricultural Sciences, 22(2): 851- 867‏.‏ https://doi.org/10.32649/ajas.2024.184461.
  16. Jasim, H. H., and Nafea, H. H. (2021). Effect of chitosan and antibiotic adding to corn-soybean diet on the productive performance of broiler chickens. Indian Journal of Ecology, 48(13): 10-14.‏
  17. Jia, L., Zhang, X., Li, X., Schilling, M. W., Peebles, E. D., Kiess, A. S., and Zhang, L. (2022). Internal organ and skeletal muscle development in commercial broilers with woody breast myopathy. Poultry Science, 101(9): 102012. https://doi.org/10.1016/j.psj.2022.102012.
  18. Khambualai, O., Yamauchi, K. E., Tangtaweewipat, S., and Cheva-Isarakul, B. (2008). Effects of dietary chitosan diets on growth performance in broiler chickens. The Journal of Poultry Science, 45(3): 206-209.‏ https://doi.org/10.2141/jpsa.45.206.
  19. Khan, I., Zaneb, H., Masood, S., Ashraf, S., Rehman, H. F., Tahir, S. K., ... and Shah, M. (2021). Supplementation of selenium nanoparticles-loaded chitosan improves production performance, intestinal morphology, and gut microflora in broiler chickens. The journal of poultry science, 59(3): 272-281.‏ https://doi.org/10.2141/jpsa.0210026.
  20. Li, J., Cheng, Y., Chen, Y., Qu, H., Zhao, Y., Wen, C., and Zhou, Y. (2019). Dietary chitooligosaccharide inclusion as an alternative to antibiotics improves intestinal morphology, barrier function, antioxidant capacity, and immunity of broilers at early age. Animals, 9(8): 493.‏ https://doi.org/10.3390/ani9080493.
  21. Mousa, B. H., Nafa, H. H., Al-Rawi, Y. T., and Al-Dulaimy, R. K. (2018). Effect of partial substitution of crude glycerol as an alternative energy source to diets in productive performance and some blood parameters of broiler. Journal of Pharmaceutical Sciences and Research, 10(11): 2907-2911.‏
  22. Nafea, H. H., and Ahmed, M. Th. (2024). Individual and synergistic effect of the addition of astaxanthin and magnolol compared with vitamin to the diet of laying hens on some physiological traits and oxidation indicators of stored eggs. Anbar Journal of Agricultural Sciences, 22(1): 719-734. https://doi.org/10.32649/ajas.2023.141634.1057.
  23. Nafea, H. H., Alkhilani, F. M., and Hamid, B. I. (2019). Effect of adding high levels of phytease enzyme to cornsoybeans based diets in the production and physiological performance of broilers. Biochemical and Cellular Archives, 19(1).‏ DOI: 10.35124/bca.2019.19.1.1621.
  24. National Research Council (NRC). (1994) Nutrient requirement of poultry, 9th revisited National Academy Press, Washington D.C., U.S.A.
  25. Qin, C., Gao, J., Wang, L., Zeng, L., and Liu, Y. (2006). Safety evaluation of short-term exposure to chitooligomers from enzymic preparation. Food and chemical toxicology, 44(6): 855-861.‏ https://doi.org/10.1016/j.fct.2005.11.009.
  26. Ramlucken, U., et al. (2020). Role of probiotics in alleviating heat stress in broiler chickens. Poultry Science, 99(9): 4505-4516.
  27. SAS Institute. (2017). SAS/STAT® 14.3 User’s Guide. Cary, NC 27513 (USA): SAS Institute.
  28. Sjofjan, O., Adli, D. N., Harahap, R. P., Jayanegara, A., Utama, D. T., and Seruni, A. P. (2021). The effects of lactic acid bacteria and yeasts as probiotics on the growth performance, relative organ weight, blood parameters, and immune responses of broiler: A meta-analysis. F1000Research, 10: 183.‏ https://doi.org/10.12688/f1000research.51219.3.
  29. Soltan, M. A., Shewita, R. S., and El-Katcha, M. I. (2008). Effect of dietary anise seeds supplementation on growth performance, immune response, carcass traits and some blood parameters of broiler chickens. Int. J. Poult. Sci, 7(11): 1078-1088.
  30. Tahir, S. K., Yousaf, M. S., Ahmad, S., Shahzad, M. K., Khan, A. F., Raza, M., ... and Rehman, H. (2019). Effects of chromium-loaded chitosan nanoparticles on the intestinal electrophysiological indices and glucose transporters in broilers. Animals, 9(10): 819.‏ https://doi.org/10.3390/ani9100819.
  31. Yazhini, P., Visha, P., Selvaraj, P., Vasanthakumar, P., and Chandran, V. (2018). Dietary encapsulated probiotic effect on broiler serum biochemical parameters. Veterinary world, 11(9): 1344.‏ https://doi.org/10.14202/vetworld.2018.1344-1348.
Statistics
Article View: 976
PDF Download: 339


Journal Management System. Powered by iJournalPro.com