Colorectal cancer is the most common gastrointestinal tract cancer worldwide. Cyclin D1 shows increased expression both in advanced adenomas , high dysplasia grade, and/or villous morphology) and colorectal adenocarcinoma with loss of expression in normal cells.
Aim of study :
To evaluate the IHC expression of Cyclin D1 in colorectal adenoma and compare it to adenocarcinoma and to correlate it with different parameters .
Materials and methods:
This retrospective study was done on tissue blocks of (40) cases (20) of which are colorectal adenomatous polyps,(20) were colorectal adenocarcinoma (covering the period from November 2018 to March 2020).
Results:
Cyclin D1 expression was lost in normal mucosa and showed variable staining, the adenoma staining percentage was score 0 in 20%(4), score I 20%(4) , score II 40%(8) and score III 20%(4) ,none had score IV While the staining percentage of colorectal adenocarcinoma was score 0 10%(2), score I 35%(7) , score II 35%(7), score III 5%(1) and score IV 15%(3) of cases.
The mean immunohistochemical staining score was significantly higher in distal colorectal than proximal adenoma (p=0.045).When adenoma cases were evaluated separately for (histopathological type ,grade, size) 2 cases (10%) had villous adenoma morphology and 9 (45%) for tubular and tubulovillous ,11 (55%) showed high grade dysplasia and 9 (45%) showed low grade dysplasia and colorectal adenoma sizes ranged between (0.3-2.5) cm 15 (75%) were less than 2 cm ,5 (25%) were equal to or larger than 2 cm.
The mean score showed a significant difference according to histopathological type (P value =0.11), grade of dysplasia (P value <0.001) and size ( P value =0.045)in colorectal adenoma.
Conclusion:
There was no significant difference in the Cyclin D1 score in colorectal adenoma and adenocarcinoma and showed a significant difference according to histopathological type , grade of dysplasia and size in colorectal adenoma. |
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