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Abundance of Capsule Associated Genes among Klebsiella pneumoniae and the relationship with biofilm formation | ||
| Journal of University of Anbar for Pure Science | ||
| Volume 19, Issue 1 - Serial Number 19, May and June 2025, Pages 23-31 PDF (1.08 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2024.151564.1285 | ||
| Authors | ||
| Dhuha S. Khaleel* 1; Xing Huang2; Thamer Yousif Mutter1 | ||
| 1Department of Biology, College of Science, University of Anbar, Ramadi, Iraq | ||
| 2Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing, China | ||
| Abstract | ||
| Klebsiella pneumoniae is a member of Enterobacteriacea that causes pneumonia and urinary tract infections especially to immune-deficiency patients. It is characterized by presence of polysaccharide capsule which is a significant factor in enhancing Klebsiella’s pathogenicity. The study is aimed to detect the abundance of capsule associated genes in Klebsiella pneumoniae strains and the relationship with biofilm formation. Antibiotic resistance profile was performed using Kirby-Bauer method and biofilm formation was assessed using crystal violet method in a 96-well microtiter plate. Gene detection in Fifty Klebsiella pneumoniae isolates were performed using PCR analysis. Four capsule associated genes, including rcsA, galF, manC, and wzi were included in the study. The results showed that high resistance profile of the isolates against most antibiotics used in this study. Biofilm formation results indicated that 39 out of the 50 isolates have the ability to form biofilm with 68% weak biofilm producer, 10% moderate producer and there was no strong biofilm producer among the 50 strains. PCR analysis results showed that rcsA (100%) was the most abundant gene as it detected in all the 50 isolates, followed by galF (90%), manC (72%), and wzi (64%). Interestingly, the four genes were highly redundant as the results showed that all four genes detected in the same strain in 24 isolate (48%). Statistical analysis indicated non-statistical significance between biofilm formation with the detected genes and the antibiotic resistance profile. In conclusion, capsule associated genes are highly abundant in local clinical Klebsiella pneumoniae and there is no correlation between those gene | ||
| Keywords | ||
| Klebsiella pneumoniae; Capsule; Associated genes; Biofilm; Resistance Profile | ||
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