- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Assessment of the Role of Coenzyme Q10 on the Spleen Histological Structure of Experimentally-induced Diabetes in Rats | ||
| Journal of University of Anbar for Pure Science | ||
| Volume 19, Issue 1 - Serial Number 19, May and June 2025, Pages 17-22 PDF (853.46 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2024.150605.1272 | ||
| Authors | ||
| Rashied M Rashied; Hajer SH. Hamed; Maryam Ibrahim Salman* ; Hala M. Hamad | ||
| Department of Biology, College of Science, University of Anbar, Ramadi, Iraq | ||
| Abstract | ||
| Diabetes is a widespread medical condition that afflicts individuals in Iraq and globally. This disease disrupts the metabolism of proteins, carbohydrates, and fats, impacting the physiology of cells throughout the body. Diabetes can lead to severe health complications, the aim of the study is to investigate the impact of diabetes on the spleen and the potential role of coenzyme Q10 treatment. The study included 20 rats categorized into four equivalent groups (5 each); the control group was the first group; CoQ10 was the second group in which animals were given Coenzyme Q10 at a dose of 10 mg/kg; the third group was diabetes group rats, which were intravenously injected with a dose of 42 mg/kg of alloxan; and treated rats were the fourth group in which rats were received alloxan with Coenzyme Q10. Treatments continued daily for two months. The study revealed that administration of alloxan resulted in a statistically significant increase (P≤ 0.05) in fasting blood sugar (FBS) levels in the serum. In addition, treatment with alloxan in rats induced histopathological changes in the spleen, including severe fatty changes and atrophy of white pulp with severe congested red pulp. co-administration of coenzyme Q10 to diabetic animals led to a significant decrease (P≤ 0.05) in serum blood glucose levels and restored the histological integrity of the spleen. CoQ10 may potentially offer a protective effect against alloxan-induced diabetes and spleen damage in rats. These findings may hold significant implications for the development of new treatment options for diabetes and related conditions. | ||
| Keywords | ||
| Alloxan; Coenzyme Q10; Diabetes mellitus; and Spleen | ||
| References | ||
|
[1] Antar SA, Ashour NA, Sharaky M, Khattab M, Ashour NA, Zaid RT, Roh EJ, Elkamhawy A, Al-Karmalawy AA. (2023). Diabetes mellitus: Classification, mediators, and complications; A gate to identify potential targets for the development of new effective treatments. Biomedicine & Pharmacotherapy. 168 115734.
[2] Poznan A, Grechko AV, Poggio P, Myasoedova VA, Alfieri V, Orekhov AN. (2020). The diabetes mellitus–atherosclerosis connection: The role of lipid and glucose metabolism and chronic inflammation, Int. J. Mol. Sci. 21 (5) :1835, https://doi.org/10.3390/ijms21051835.
[3] Sakitani K, Enooku K, Kubo H, Tanaka A, Arai H, Kawazu S, Koike K. (2017). Clinical characteristics of patients with diabetes mellitus and fatty liver diagnosed by liver/spleen Hounsfield units on CT scan. J Int Med Res. Jun;45(3):1208-1220.
[4] Cesta MF. (2006). Normal structure, function, and histology of the spleen. Toxicol Pathol.;34(5):455-65.
[5] Martelli A, Testai L, Colletti A, Cicero AF. (2020) Coenzyme Q10: Clinical applications in cardiovascular diseases. Antioxidants. Apr;9(4):341.
[6] Bentinger M, Brismar K, Dallner G. (2007).The antioxidant role of coenzyme Q. Mitochondrion. Jun 1;7: S41-50.
[7] Saini R. (2011).Coenzyme Q10: The essential nutrient. Journal of Pharmacy and Bioallied Sciences. Jul 1;3(3):466-7.
[8] Bhagavan HN, Chopra RK. (2006). Coenzyme Q10: absorption, tissue uptake, metabolism and pharmacokinetics. Free radical research. Jan 1;40(5):445-53.
[9] Queiroz LA, Assis JB, Guimarães J, Sousa ES, Milhomem AC, Sunahara KK, Sá-Nunes A, Martins JO.(2021). Endangered lymphocytes: The effects of alloxan and streptozotocin on immune cells in type 1 induced diabetes. Mediators of Inflammation. Oct 19;2021.
[10] Lenzen S. (2008). The mechanisms of alloxan-and streptozotocin-induced diabetes. Diabetologia. Feb;51(2):216-26.
[11] Macdonald Ighodaro O, Mohammed Adeosun A, Adeboye Akinloye O. (2017). Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina. Dec;53(6):365-74.
[12] Szkudelski T. (2001). The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological research. Jan 1;50(6):537-46.
[13] Salman MI, Rashied RM, Hamad HM, Hamad HS.(2020) The protective effect of coenzyme Q10 on experimental diabetic nephropathy in male rats. Eurasia J. Biosci. Aug 1; 14: 6883-8.
[14] Rashied RM, Salman MI, Hamad HS, Hamad HM.(2020). EFFECT OF COENZYME Q10 AGAINST ALLOXAN-INDUCED DIABETES AND LIVER TOXICITY IN MALE RATS. Biochemical & Cellular Archives. Oct 1;20(2).
[15] Reeves PG. (1997). Components of the AIN-93 diets as improvements in the AIN-76A diet. J. Nutr. . 127: 838S-841S.
[16] Lucchesi AN, Cassettari LL, Spadella CT. (2015). Alloxan-induced diabetes causes morphological and ultrastructural changes in rat liver that resemble the natural history of chronic fatty liver disease in humans. Journal of diabetes research. Feb 19;2015.
[17] Aledani AHE, Khudhair NA, Alrafas HR. (2020). EFFECT OF DIFFERENT METHODS OF ANESTHESIA ON PHYSIO-BIOCHEMICAL PARAMETERS IN LABORATORY MALE RATS. Bas.J.Vet.Res. 19(1): 206-214.
[18] Ebaid H, Al-Tamimi J, Metwalli A, Allam A, Zohir K, Ajarem J, Rady A, Alhazza IM, Ibrahim KE. (2015). Effect of STZ-induced diabetes on spleen of rats: Improvement by camel whey proteins. Pakistan J. Zool. Aug 1;47(4):1109-16.
[19] Sainio-Pöllänen S, Erkkilä S, Alanko S, Hänninen A, Pöllänen P, Simell O. (1998).The role of Fas ligand in the development of insulitis in nonobese diabetic mice. Pancreas. Mar 1;16(2):154-9.
[20] Park GB, Kim YS, Lee HK, Cho DH, Kim D, Hur DY. (2013). CD80 (B7. 1) and CD86 (B7. 2) induce EBV-transformed B cell apoptosis through the Fas/FasL pathway. International journal of oncology. Nov 1;43(5):1531-40.
[21] Jafar N, Edriss H, Nugent K. (2016). The effect of short-term hyperglycemia on the innate immune system. The American journal of the medical sciences. Feb 1;351(2):201-11.
[22] Abu-Ashour W, Twells LK, Valcour JE, Gamble JM.(2018). Diabetes and the occurrence of infection in primary care: a matched cohort study. BMC infectious diseases. Dec;18:1-8.
[23] Hussain A, Bhowmink B, Moreira NC. (2020) COVID-19 and diabetes: Knowledge in progress. diabetes research and clinical practice. April;(162):108142.
[24] Novoselova EG, Glushkova OV, Lunin SM, Khrenov MO, Parfenyuk SB, Novoselova TV, Sharapov MG, Novoselov VI, Fesenko EE. (2020). Peroxiredoxin 6 attenuates alloxan-induced type 1 diabetes mellitus in mice and cytokine-induced cytotoxicity in RIN-m5F beta cells. Journal of Diabetes Research. Aug 25;2020.
[25] Manna P, Sil PC. (2012). Arjunolic acid: beneficial role in type 1 diabetes and its associated organ pathophysiology. Free Radical Research. Jul 1;46(7):815-30.
[26] Kumar R, Kumari A, Singh JK, Nath A, Ali M, Sinha S, Kumar A. (2013).Protective effect of zingiber officinale on spleen of diabetic guinea pig. International Journal of Pharmaceutical Science Invention. Mar;2(3):32-7.
[27] Wojnar W, Zych M, Kaczmarczyk-Sedlak I. (2018). Antioxidative effect of flavonoid naringenin in the lenses of type 1 diabetic rats. Biomedicine & Pharmacotherapy. Dec 1; 108: 974-84.
[28] Czerwińska ME, Gąsińska E, Leśniak A, Krawczyk P, Kiss AK, Naruszewicz M, Bujalska-Zadrożny M. (2018). Inhibitory effect of Ligustrum vulgare leaf extract on the development of neuropathic pain in a streptozotocin-induced rat model of diabetes. Phytomedicine. Oct 1; 49: 75-82.
[29] Tsuneki H, Sekizaki N, Suzuki T, Kobayashi S, Wada T, Okamoto T, Kimura I, Sasaoka T. (2007). Coenzyme Q10 prevents high glucose-induced oxidative stress in human umbilical vein endothelial cells. European Journal of Pharmacology. Jul 2;566(1-3):1-10. | ||
|
Statistics Article View: 549 PDF Download: 416 |
||