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Efficient Delivery of Recombinant Human Bone Morphogenetic Protein (Rhbmp-2) With Cockle Shell Derived Calcium Carbonate Nanoparticles (CaCO3NPs) | ||
| Tikrit Journal for Dental Sciences | ||
| Volume 11, Issue 2, December 2023, Pages 216-231 PDF (915.97 K) | ||
| DOI: 10.25130/tjds.11.2.8 | ||
| Authors | ||
| Ataa T. Ghazi1; Hayder F. Saloom2; Rana I. Mahmood* 3 | ||
| 1Department of Orthodontics, College of Dentistry, University of Baghdad, Baghdad, Iraq | ||
| 2P.O.P Department, College of Dentistry, Mustansiriya University, Baghdad,Iraq | ||
| 3Dep. of Biomedical Engineering College of Engineering, AL-Nahrain University Baghdad, Iraq | ||
| Abstract | ||
| Bone morphogenetic protein-2 (BMP-2) has a significant function in the formation of cartilage and bones. Notably, dosing of only BMP-2 protein intravenously is ineffective. Persistent transportation of the stabilized BMP-2 through a carrier has been seen to be essential for enhancing the osteogenesis impact of BMP-2. The current research built a new system of drug delivery by utilising cockle shell derived calcium carbonate nanoparticles (CaCO3NPs) and studied the efficacy of the delivery system on the recombinant human bone morphogenetic protein (rhBMP-2). rhBMP-2-CaCO3NPs nanoparticles were synthesised by means of a modest precipitation procedure along with mechanical grinding. Fourier-transform infrared spectroscopy, UV–Vis spectrophotometer, scanning electron microscope, X-ray powder diffraction, transmission electron microscope, and zeta potential were utilised for characterising the conjugated rhBMP-2-CaCO3NPs. Cytotoxicity of rhBMP-2, CaCO3NPs and rhBMP-2-CaCO3NPs was studied by utilising methylthiazol tetrazolium assay against fibroblast (Rat-1) cells in comparison to rhBMP-2 and CaCO3NPs. The outcomes signified bio-stability of CaCO3NPs and lower toxicity for Rat-1 cells. In summary, CaCO3NPs were prepared by a simple precipitation process. The ensuing nanoparticles could competently entrap rhBMP-2 and generated stable rhBMP-2-CaCO3NPs. A sustained discharge of rhBMP-2 from the CaCO3NPs was seen. CaCO3NPs loaded with rhBMP-2 demonstrated reasonable bio-compatibility. The outcomes indicated that CaCO3NPs may have significant ability as carrier of therapeutic proteins within bone tissue engineering. | ||
| Keywords | ||
| Orthodontics CaCO3NPs rhBMP; 2 drug deliver | ||
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