T. F. Alkhamisi, F., H. Al-Issawi, M. (2025). GENETIC DIVERSITY OF SOME BREAD WHEAT GENOTYPES USING RAPD TECHNIQUE. , 23(1), 770-778. doi: 10.32649/ajas.2025.160071.1681
F. T. F. Alkhamisi; M. H. Al-Issawi. "GENETIC DIVERSITY OF SOME BREAD WHEAT GENOTYPES USING RAPD TECHNIQUE". , 23, 1, 2025, 770-778. doi: 10.32649/ajas.2025.160071.1681
T. F. Alkhamisi, F., H. Al-Issawi, M. (2025). 'GENETIC DIVERSITY OF SOME BREAD WHEAT GENOTYPES USING RAPD TECHNIQUE', , 23(1), pp. 770-778. doi: 10.32649/ajas.2025.160071.1681
T. F. Alkhamisi, F., H. Al-Issawi, M. GENETIC DIVERSITY OF SOME BREAD WHEAT GENOTYPES USING RAPD TECHNIQUE. , 2025; 23(1): 770-778. doi: 10.32649/ajas.2025.160071.1681

GENETIC DIVERSITY OF SOME BREAD WHEAT GENOTYPES USING RAPD TECHNIQUE

Anbar Journal of Agricultural Sciences
Volume 23, Issue 1, June 2025, Pages 770-778    PDF (497.94 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2025.160071.1681
Authors
F. T. F. Alkhamisi* 1; M. H. Al-Issawi2
1University of Anbar, College of Agriculture, Department of Plant Protection, Anbar, Iraq
2University of Anbar, College of Agriculture, Department of Field Crops, Anbar, Iraq
Abstract
A Group of five bread wheat genotypes that were newly introduced to Iraq were assessed for their genetic similarity using the random amplified polymorphic DNA (RAPD) technique. The genomic DNA of the genotypes was amplified with five RAPD primers. Used primers produced total amplified bands of 63 out of which 25 found to be polymorphic presenting polymorphism percentage of 39.7%. Amplified fragments per primer reached a maximum of 16 bands produced by primer OPF-10 resulting in 25% polymorphism. Using cluster analysis, the genotypes were distributed into two sub-clusters; the first (A) including a single genotype (G5), while the other 4 were grouped in the second sub-cluster (B) which divided into two subgroups. Similarity values showed G1 and G3 as the closest genotypes (97%), while G5 and G3 had the lowest value of similarity with 44%. Genotype G1 showed a unique band sized 3000bp using primer OPP-05, same band size appeared in G3 using OPA-06, while G5 had a unique band with size of 200bp using OPP-5. This study highlighted the genetic similarity among the five introduced genotypes showing their potential importance as promising genetic material for wheat breeding programs in Iraq to develop wheat germplasms considering further genetic similarity investigation should be followed for the promising genotypes
Keywords
RAPD; Wheat; Genetic diversity; Breeding; Genotype
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