Estimation of Lycopene and Beta-Carotene Contents in Different Tomato Plant Tissues

Mohammed, Amjad A.; Ali, Mashhad Muhammad

doi:10.33899/rjs.2025.158704.1434

	Estimation of Lycopene and Beta-Carotene Contents in Different Tomato Plant Tissues
Rafidain Journal of Science
Articles in Press, Accepted Manuscript, Available Online from 02 August 2025
Document Type: Research Paper
DOI: 10.33899/rjs.2025.158704.1434
Authors
Amjad A. Mohammed^* ¹; Mashhad Muhammad Ali²
¹Department of Biology/ College of Science/ Mosul University/ Iraq
²Department of Biology, College of Science, University of Mosul, Iraq
Abstract
Present study succeeded in induced the callus from hypocotyledon stem segments of tomato (Solanum lycopersecum) seedlings and produced its cultures then differentiated to vegetative shoot from them. The extraction and estimation of the lycopene and beta-carotene contents of these tissues were followed. The results indicated that callus was inducible from these cuttings on solid Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 after 7 days at 100%, with aggregate formed on the cuttings then its become large in size after 12 days, its separated and transferred to a medium with the same components above produced callus cultures due to the rapid and continuous division of cells. What distinguished the study was the spontaneous differentiation of vegetative shoot from callus after the second subcultured at a rate of 85%. The methods of preparing the extracts proved successful and the quantities of lycopene and beta-carotene were estimated. The best tissue in their produced was the callus, which reached 3.615 mg100 g-1 and 1.301 mg 100 g-1 at the third hour of extraction, respectively.
Keywords
Solanum lycopersecum; Callus; Differentiation; Lycopene; Beta-Carotene

Statistics Article View: 120