Detection of respiratory viruses by Enzyme-Linked Immunosorbent Assay from respiratory patients in Mosul City, Iraq

ALtaie, Anmar Ahmed; Mahmood, Mahmood N

doi:10.33899/rjs.2025.158724.1436

	Detection of respiratory viruses by Enzyme-Linked Immunosorbent Assay from respiratory patients in Mosul City, Iraq
Rafidain Journal of Science
Articles in Press, Accepted Manuscript, Available Online from 02 August 2025
Document Type: Research Paper
DOI: 10.33899/rjs.2025.158724.1436
Authors
Anmar Ahmed ALtaie^* ¹; Mahmood N Mahmood²
¹university of Mosul /college of science /biology department
²Department of Biology, College of Science, University of Mosul, Mosul, Iraq
Abstract
Respiratory tract infections (RTIs) are an important source of disease and mortality, especially in high-risk populations. Respiratory virus diagnosis must be accurate and timely to give optimum patient care and control infection. The enzyme-linked immunosorbent assay (ELISA) is a rapid assay that can detect the antigen/antibodies reaction of the respiratory pathogens in a diagnostic test. A total of 150 serum samples were collected from RTI patients who were admitted to Mosul hospitals between October and December 2023. The inclusion criteria were RTI diagnosed clinically with fever, cough, or shortness of breath. Exclusion criteria were bacterial infection confirmed or non-viral respiratory infection. The most common detected virus was Human Respiratory Syncytial Virus (HRSV) with (20 cases, 13.3%), followed by Human Rhinovirus (HRV)with (14 cases, 9.3%), Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) with (12 cases, 8.0%) and Human Enterovirus (HEV)with (10 cases, 6.6%). The other viruses detected were Influenza A Virus (IAV) with (8 cases, 5.3%), Human Metapneumovirus (HMPV) with (7 cases, 4.6%), Human Coronavirus OC43 (HCoV-OC43) with (4 cases, 2.6%), and Human Coronavirus NL63 (HCoV-NL63) with (3 cases, 2.0%). Age group analysis established that HRSV was greatest in individuals between 60-69 and 20-29 years and that the greatest cases of HRV were in the 40-49 age group. Females presented a greater infection when compared to males. The study highlights the existence of HRSV, HRV, and SARS-CoV-2 as prevailing viral etiologies of RTIs in Mosul.
Keywords
Respiratory tract infections; Rhinovirus; Metapneumovirus; Influenza A; Coronavirus

Statistics Article View: 104