Bioactive Metabolites in a New Strain of Streptomyces griseus RH250From Marl Clay in Mosul/Iraq

Khalaf, Rawaa Ahmed; Ibrahim, Hiyam Adel

doi:10.33899/rjs.2025.159208.1447

	Bioactive Metabolites in a New Strain of Streptomyces griseus RH250From Marl Clay in Mosul/Iraq
Rafidain Journal of Science
Articles in Press, Accepted Manuscript, Available Online from 02 August 2025
Document Type: Research Paper
DOI: 10.33899/rjs.2025.159208.1447
Authors
Rawaa Ahmed Khalaf^* ; Hiyam Adel Ibrahim
Department of Biology, College of Science, University of Mosul
Abstract
ABSTRACT Identification of the bioactive secondary metabolites of a crude butanol extract from a locally isolated strain of Streptomyces griseus RH250 isolated from Marl clay in Mosul / Iraq . This new strain possesses the ability to produce various kinds of metabolites, It was screened for it is antibacterial activity against Gram-negative (Alcaligenes faecalis , Pseudomonas fluorescens, Serratia marcescens, and Escherichia coli) and Gram-positive (Staphylococcus aureus) . Screening was performed using the agar-cross streak method, and the growth inhibition line was measured in millimeters. This strain has shown potent in vitro antibacterial activities against Staphylococcus aureus ( 25 mm) only . The agar diffusion well technique for crude N-butanol extract was performed . Gas chromatography-mass–mass spectrometry was used to determine bioactive secondary metabolites in crude butanol extract . This analysis showed that twenty one bioactive metabolite compounds were isolated from this strain, which have different chemical structures and biological activities.
Keywords
biological activity; cross streak; crude butanol extract; Streptomyces griseus RH250

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