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Isolation of bacteria from bacteremia, molecular diagnosis, and investigating their relationship with kidney functions | ||
| Rafidain Journal of Science | ||
| Articles in Press, Accepted Manuscript, Available Online from 02 August 2025 | ||
| Document Type: Review Paper | ||
| DOI: 10.33899/rjs.2025.159823.1452 | ||
| Author | ||
| Rusliya Younis Mahmoud* | ||
| University of Mosul/College of Science/ Department of Life Sciences | ||
| Abstract | ||
| The blood infection, known as bacteremia, is considered a life-threatening condition and critical infection. In this study, 250 blood samples were collected from various hospitals in Mosul (including both male and female patients of different ages). Blood was drawn from patients suspected of being infected with bacteremia and was directly cultured in brain-heart infusion broth and incubated aerobically, which was considered the primary culture. After observing any changes in the medium the following day, a secondary culture was performed on two media: basal blood agar and MacConkey agar. The colony morphologies were observed, and the cells were examined using Gram staining. Molecular diagnosis was then carried out based on the 16S rRNA diagnostic gene. Following nucleotide sequencing and comparison with global strains in the National Center for Biotechnology Information (NCBI) database, a total of 19 Gram-positive bacterial strains were identified, accounting for 7.6% of the samples, and 16 Gram-negative bacterial strains, accounting for 6.4%. The isolated bacteria were as follows: Klebsiella pneumoniae 31.5%, Shigella sonnei 26.3%, Serratia surfactantfaciens 10.5%, Pseudomonas aeruginosa 10.5%, Burkholderia paludis 5.2%, Escherichia coli 10.5%, Enterobacter 5.2%, Staphylococcus aureus 12.5%, Staphylococcus haemolyticus 12.5%, Staphylococcus epidermidis 68.7%, and Micrococcus yunnanensis 6.2% The bacterial subtype of Klebsiella pneumoniae was identified through the use of the focus broth test and malonate consumption test. | ||
| Keywords | ||
| bacteremia; Klebsiella subspepcies; urea; creatinine | ||
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