The Effect of Magnetic Field on The Thymol Compound Content of the Thymus vulgaris L. Callus Tissues.

Yahay, Rana Tariq

doi:10.33899/rjs.2025.159939.1456

	The Effect of Magnetic Field on The Thymol Compound Content of the Thymus vulgaris L. Callus Tissues.
Rafidain Journal of Science
Articles in Press, Accepted Manuscript, Available Online from 02 August 2025
Document Type: Research Paper
DOI: 10.33899/rjs.2025.159939.1456
Author
Rana Tariq Yahay^*
Department of Biophysics/ College of Science/ University of Mosul
Abstract
This study demonstrated the positive effect of the magnetic field (MF) on the germination of thyme Thymus vulgaris L. Seeds, growth its seedlings and initiate and grow the callus from its stem parts. The results showed the success of cultured seedling stem pieces on Murashige and Skoog (MS) medium supported by Kinetin and 2,4-Dichlorophenoxy acetic acid (2,4-D) with 0.5 and 1.0 mg/L respectively in high percentage of callus initiation, reaching 80%. The Exposing thyme seeds to the magnetic field led to giving a high percentage of germination and growth to form vital seedlings, Also, when exposing its stem pieces to MF for different periods led to an increase in the callus initiation percentage, reaching its highest at a period of 60 minutes 95% for initiated. The study also proved the effective role of the MF in stimulating the growth of callus cells to grow reaching to produce the secondary metabolic compounds by increasing the callus cells’ oil content, accompanied by an increase in the percentage of thymol (the main component of thyme oil, which has medicinal benefits) recording the highest oil content of 3.49% and thymol content of 42.01% for callus cells exposed to MF at 60 minutes compared to the unexposed callus group (control) with 1.94% callus oil content and 38.98% thymol.
Keywords
Magnetic field (MF); thyme (Thymus vulgaris); callus; oil content; thymol compound

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