Laboratory experiments were carried out in the postgraduate laboratories, Biology Department – College of Education / Kerbala University . The study aimed to determine contamination extent of some foodstuff like wheat Triticum aestivum , corn Zea mays , groundnut Arachis hypogaea , pistachio Pistacia vera , honey pumpkin grain Cucurbita moschata and sunflower grain Helianthus annus with fungi, particularly Aspergillus flavus and the collections of the General Company for Grain Trade / Branch of Karbala, Al-Dehan market , the wholesale and local markets . Also determination of isolates fungus A. flavus has been done which obtained from this study in the production of aflatoxin B1 and B2.
The results showed that 547 fungal isolates were found included (Alternaria alternata , Aspergillus flavus , Aspergillus niger , Aspergillus terreus , Cladosporium cladosporsis ,Fusarium oxyporium ,Penicillium digitatum , Penicillium spinulosum , Penicillium spp and Rhizopus spp) More contaminated sites with fungal were Al-Dehan market with 216 isolates , and the wholesale market has the second highest location in contamination with 155 isolates, while the local market recorded 122 isolates, grain stores came in the last level among the sites with 54 isolate. These crops were not different in their content from genera and species , but they differed in quantity, where the corn reached the highest contamination of fungi with 123 isolates , the wheat had the lowest percentage of the wholesale number 54 isolates , while honey pumpkin grain recorded the second largest proportion reached to 110 isolates , followed by groundnut, sunflower grain and pistachio which recorded( 96, 90 and 74 ) isolates , respectively. The first fungus A. flavus that recorded 99 isolates at a percentage of 18.1% , while other fungi recorded lower rates.
Eighteen isolates of the fungus A. flavus only 11 isolates can produce aflatoxin B1 and B2 at a rate of 61.2%, while 7 with 38.8%were non-produced aflatoxin B1 and B2. |