AL-Warshan, S., Mizil, F., Hadi, S. (2025). Detection of Fungi Producing Aflatoxin B1 and Ochratoxin A in Poultry Feed in Anbar Governorate. , 23(2), 1433-1445. doi: 10.32649/ajas.2025.189316
S. H. S. AL-Warshan; F. K. Mizil; S. Th. Hadi. "Detection of Fungi Producing Aflatoxin B1 and Ochratoxin A in Poultry Feed in Anbar Governorate". , 23, 2, 2025, 1433-1445. doi: 10.32649/ajas.2025.189316
AL-Warshan, S., Mizil, F., Hadi, S. (2025). 'Detection of Fungi Producing Aflatoxin B1 and Ochratoxin A in Poultry Feed in Anbar Governorate', , 23(2), pp. 1433-1445. doi: 10.32649/ajas.2025.189316
AL-Warshan, S., Mizil, F., Hadi, S. Detection of Fungi Producing Aflatoxin B1 and Ochratoxin A in Poultry Feed in Anbar Governorate. , 2025; 23(2): 1433-1445. doi: 10.32649/ajas.2025.189316

Detection of Fungi Producing Aflatoxin B1 and Ochratoxin A in Poultry Feed in Anbar Governorate

Anbar Journal of Agricultural Sciences
Volume 23, Issue 2, December 2025, Pages 1433-1445    PDF (514.25 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2025.189316
Authors
S. H. S. AL-Warshan1; F. K. Mizil2; S. Th. Hadi3
1Department of Plant Protection, College of Agriculture, University of Anbar
2Vocational Education Department, Rusafa 2, Ministry of Education
3Department of Food Sciences, College of Agriculture, University of Anbar
Abstract
The study was conducted at the Department of Plant Protection, College of Agriculture, Anbar University to detect toxigenic fungi contaminating poultry feed and the concentrations of aflatoxin B1 (AFB1) and ochratoxin A (OTA) in them. The results showed the feed contaminated with various fungi, the most prevalent being Aspergillus spp at 57%, followed by Penicillium spp, Fusarium spp, and Mucor spp at 26, 9 and 3%, respectively. Colorimetric analysis on thin layer chromatography (TLC) plates revealed that isolates producing AFB1 formed 66.66% of the total number of isolates while it was 100% for OTA. AFB1 detection in poultry samples showed that the highest concentration of the toxin was in samples from the city of Hit at 50-120 ppb, 30-75 ppb in those from Saqlawiyah, and 40-60 ppb in samples from Fallujah and Al-Qa`im city. As for OTA, the results showed lower contamination levels than for AFB1 at 45-50 ppb in samples from the city of Al-Karmah, and 30-40 ppb in Ramadi and Al-Qa`im.  Samples from Fallujah city recorded the lowest OTA concentration at 30-40 ppb while none was detected in feed samples from the city of Saqlawiyah, Hit, and Ana
Keywords
Fungi; Aflatoxin B1; Ochratoxin A; Feed; Poultry
References
  1. Ahmad, T., Wang, S., and Liu, Y. (2025). Aspergillus flavus and Aflatoxins. Toxins, 17(7): 326. https://doi.org/10.3390/toxins17070326.
  2. Al-Fahdawi, S. T. A., and Al-Warshan, S. H. S. (2024). Effect of some plant extracts on the growth of fungus Aspergillus flavus and reduce the production of aflatoxin B1 on stored corn. Anbar Journal of Agriculture Science, 22(1): 735-749. https://doi.org/10.32649/ajas.2024.183833.
  3. Al-Shuhaib, M. B. S., Albakri, A. H., Hashim, H. O., Alwan, S. L., Almandil, N. B., Selvaraj, P., ... and Francis Borgio, J. (2020). High prevalence of Aflatoxin B1 in Aspergillus flavus infecting stored rice grains. Journal of Agricultural Science and Technology, 22(3): 737-745. https://dor.isc.ac/dor/20.1001.1.16807073.2020.22.3.18.8.
  4. Attia, Y. A., Rahman, M. T., Hossain, M. J., Basiouni, S., Khafaga, A. F., Shehata, A. A., and Hafez, H. M. (2022). Poultry production and sustainability in developing countries under the COVID-19 crisis: Lessons learned. Animals, 12(5): 644. https://doi.org/10.3390/ani12050644.
  5. Barnett, H. L., and Hunter, B. B. (1998). Illustrated genera of imperfect fungi. 4th, APS Press, St. Paul, 218.
  6. Daliri, A., Shams-Ghahfarokhi, M., and Razzaghi-Abyaneh, M. (2023). Detection of Aflatoxin B1-producing Aspergillus flavus strains from pistachio orchards soil in Iran by multiplex polymerase chain reaction method. Current Medical Mycology, 9(3): 1. https://doi.org/10.22034/CMM.2023.345055.1420.
  7. Daou, R., Joubrane, K., Maroun, R. G., Khabbaz, L. R., Ismail, A., and El Khoury, A. (2021). Mycotoxins: Factors influencing production and control strategies. AIMS Agriculture and Food, 6(1): 416-447. https://doi.org/10.3934/agrfood.2021025.
  8. Deligeorgakis, C., Magro, C., Skendi, A., Gebrehiwot, H. H., Valdramidis, V., and Papageorgiou, M. (2023). Fungal and toxin contaminants in cereal grains and flours: systematic review and meta-analysis. Foods, 12(23): 4328. https://doi.org/10.3390/foods12234328.
  9. Egan, H., Stoloff, L., Scott, P., Castegnarro, M., O'Neill, I. K., and Bartscch, H. (1982). Environmental carcinogens selected methods of analysis. Volume 5, Some Mycotoxins, Analytical Method. International Agency for Research on Cancer. Lyon.

10.     Gurikar, C., Shivaprasad, D. P., Sabillón, L., Gowda, N. N., and Siliveru, K. (2023). Impact of mycotoxins and their metabolites associated with food grains. Grain and Oil Science and Technology, 6(1): 1-9. https://doi.org/10.1016/j.gaost.2022.10.001. 

  1. Hafez, H. M., and Attia, Y. A. (2020). Challenges to the poultry industry: current perspectives and strategic future after the COVID-19 outbreak. Frontiers in veterinary science, 7: 516. https://doi.org/10.3389/fvets.2020.00516.
  2. Imade, F., Ankwasa, E. M., Geng, H., Ullah, S., Ahmad, T., Wang, G., ... and Liu, Y. (2021). Updates on food and feed mycotoxin contamination and safety in Africa with special reference to Nigeria. Mycology, 12(4): 245-260. https://doi.org/10.1080/21501203.2021.1941371.
  3. Kemboi, D. C., Ochieng, P. E., Antonissen, G., Croubels, S., Scippo, M. L., Okoth, S., ... and Gathumbi, J. K. (2020). Multi-mycotoxin occurrence in dairy cattle and poultry feeds and feed ingredients from Machakos Town, Kenya. Toxins, 12(12): 762. https://doi.org/10.3390/toxins12120762.
  4. Khan, R., Anwar, F., and Ghazali, F. M. (2024). A comprehensive review of mycotoxins: Toxicology, detection, and effective mitigation approaches. Heliyon, 10(8). https://doi.org/10.1016/j.heliyon.2024.e28361.
  5. Klich, M. A. (2002). Identification of common Aspergillus species. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands.
  6. Krnjaja, V., Stojanovic, L. J., Trenkovski, S., Bijelic, Z., and Tomasevic, D. (2010). The frequency of pathogenic fungi genera in poultry feed. Journal of Food, Agriculture and Environmental. 8(3and4): 589-591. https://doi.org/10.54865/mjas111187k.
  7. Kutama, A. S., Adamu, M., Sani, M. D., and Mai–Abba, A. (2022). Isolation and identification of aflatoxin producing fungi from different foodstuffs at Shuwarin Market, Jigawa State, Nigeria. Dutse Journal of Pure and Applied Sciences, 8(1b): 9-15. https://doi.org/10.4314/dujopas.v8i1b.2.
  8. Mohammed, H. H., Ismaiel, A., and EL-Sayed, M. T. (2023). Isolation and identification of ochratoxigenic fungi from food and feed commodities in Sharika governorate. Bulletin of Faculty of Science, Zagazig University, 2023(3): 1-10. https://doi.org/10.21608/bfszu.2023.178711.1218.
  9. Rahim, H. M., Othman, D. H., Majeed, R. K., Saeed, N. M., and Ismaeel, D. O. (2020). Mycotoxins in poultry feed and feed ingredients in Sulaymaniyah, Kurdistan region of Iraq. Basra Journal of Veterinary Research. 19(2): 210-222.
  10. Rajabu, R. A., and Chilipweli, P. M. (2024). Revocatus Mang'ara Mycotoxin Contamination in Cereal Grains and Associated Risk Factors: A Case Study of Iringa Municipality. Tanzania. Journal of Food Science and Nutrition Research, 7: 214-222. DOI:10.26502/jfsnr.2642-110000168.
  11. Rajarajan, P. N., Rajasekaran, K. M., and Devi, N. A. (2013). Aflatoxin contamination in agricultural commodities. Indian Journal of Pharmaceutical and Biological Research, 1(4): 148-151. https://doi.org/10.30750/ijpbr.1.4.25.
  12. Tokarska-Rodak, M., Zarębska, M., and Nitychoruk, D. K. (2022). Mycotoxin Contaminants of Walnuts-Preliminary Studies. Polish Journal of Environmental Studies, 31(1): 493-498. DOI: 10.15244/pjoes/138990.
  13. Vlaicu, P. A., Untea, A. E., and Oancea, A. G. (2024). Sustainable poultry feeding strategies for achieving zero hunger and enhancing food quality. Agriculture, 14(10): 1811. https://doi.org/10.3390/agriculture14101811.
  14. Xue, M., Qu, Z., Moretti, A., Logrieco, A. F., Chu, H., Zhang, Q., ... and Zhu, Y. G. (2025). Aspergillus mycotoxins: The major food contaminants. Advanced Science, 12(9): 2412757. https://doi.org/10.1002/advs.202412757.
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