- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Evaluation Level of Gene Expression for PRE gene on Preptin Hormone in Patients with Osteoporosis in Mosul City | ||
| Rafidain Journal of Science | ||
| Articles in Press, Accepted Manuscript, Available Online from 03 September 2025 | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/rjs.2025.162656.1489 | ||
| Authors | ||
| Elaf Gh. Nafi* ; Saba Zeki Al-Abachi | ||
| Department of Chemistry, Collage of Science, University of Mosul, Mosul, Iraq, | ||
| Abstract | ||
| Osteoporosis is often referred to as a silent disease because it typically develops without noticeable symptoms. This condition, characterized by a decrease in bone density, has become more widespread, leading to sudden fractures in patients without any prior warning. It has been increasingly observed in people under the age of 35, prompting researchers to explore genetic factors. Hormones that regulate nutritional status, including preptin, play a significant role in bone metabolism. Preptin is a recently discovered 34-amino acid peptide hormone, secreted alongside insulin and amylin by pancreatic beta cells. Given its potential link to osteoporosis, the hormone preptin was selected for further genetic study. Blood samples were collected in EDTA tubes for DNA extraction and in triazole tubes for RNA extraction. Gene expression of preptin was analyzed in blood samples from osteoporosis and osteopenia patients in Mosul, Iraq, and compared to healthy individuals, due to the high incidence of osteoporosis and its genetic and hereditary associations.The study aimed to assess the genetic variation of preptin genes in 40 blood samples from patients aged 35 to 75, who were receiving ongoing treatment at Ibn Sina Teaching and Al-Jomhory hospitals. A control group of 30 healthy individuals of similar age was also included. Additionally, the research focused on detecting genetic mutations in the human DNA sequence, which has been registered in the International Gene Bank under number LC859842. | ||
| Keywords | ||
| Preptin Hormone; Gene expression; Sequence DNA; Osteoporosis | ||
|
Statistics Article View: 101 |
||