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Using of milk ELISA test to investigate the presence of antibodies resulting from vaccination of ewes with Brucella melitensis strain Rev.1 vaccine | ||
| Iraqi Journal of Veterinary Sciences | ||
| Volume 23, Supplement 1, June 2009 PDF (1.61 M) | ||
| Document Type: Conference Paper | ||
| DOI: 10.33899/ijvs.2009.189466 | ||
| Author | ||
| W. S. Al-Khafaji | ||
| Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, Iraq | ||
| Abstract | ||
| This study was included 18 of native lactating ewes divided into three equal groups which was vaccinated with Rev.1 vaccine. The sensitivity of Rose Bengal test (RB) was 50% ,50% and 0% in the 4th day postvaccination in animals of 1st group which vaccinated with dose 1×109 colony forming units(CFU)/ml via subcutaneous route, the 2nd group vaccinated with dose 1×109 CFU/ml via conjunctival route the 3rd group vaccinated with dose 1×107 CFU/ml via subcutaneous route respectively, then increased to reach 100% in days 7, 30 and 15 in vaccinated animal groups 1st, 2ndand 3rd respectively and continue till the final of experiment, the sensitivity of serum agglutination test (SAT) reached 100% in 7th and 15th day in 1st and 3rd groups respectively, while, it reached the highest value 80% in 30th day in 2nd group. The milk ELISA showed sensitivity reached 100% from the starting of experiment till the final in the 60 day postvaccination in all vaccinated groups, the results of SAT revealed the highest antibody titers was in the 1st and 3rd then 2nd groups. also showed significant correlation between optical density (O.D.) values of milk ELISA and the results of SAT while the correlation coefficient value was r= 0.80 at the level P<0.05. The results of bacterial culture was negative for milk and vaginal swabs of ewes in all vaccinated groups. | ||
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