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Diagnosis of Clostridium perfringens strains isolated from enterotoxemia cases in sheep using polymerase chain reaction (PCR) technique | ||
| Iraqi Journal of Veterinary Sciences | ||
| Volume 23, Supplement 1, June 2009 PDF (1.23 M) | ||
| Document Type: Conference Paper | ||
| DOI: 10.33899/ijvs.2009.189524 | ||
| Authors | ||
| M. A. Hamad1; N. Habra2; A. Kalb-Allouz3 | ||
| 1Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq | ||
| 2Department of Microbiology, College of Veterinary Medicine, AL-Baath University, Syria | ||
| 3Department of Animal Diseases, College of Veterinary Medicine, AL-Baath University, Syria | ||
| Abstract | ||
| This study included diagnosis and typing of 100 bacterial isolates of Clostridium perfringens that isolated from enterotoxaemia cases in sheep in Hama province by using Multiplex PCR. The genotyping of these isolates indicated that all isolates were Clostridium perfringens and 47 of them were type A (47 %), 38 were type D (38%), and 15 were type C (15%), with obvious predominating for type A strains which deserve the attention to this type as a one of major causes of enterotoxaemia in sheep. Multiplex PCR technique can be used in diagnosis and typing of C. perfringens strains instead of conventional procedures. | ||
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