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Prevalence, Histopathological Diagnosis, and Molecular Identification of Cystic Echinococcosis in Slaughtered Sheep and Goats of Duhok, Iraq | ||
| Basrah Journal of Veterinary Research | ||
| Article 8, Volume 24, Issue 3, September 2025, Pages 82-97 PDF (784.31 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvr.2025.161282.1228 | ||
| Authors | ||
| Zuzan Nawzad Hussein* 1; Mohammed Shukri Shukur2 | ||
| 1Department of Medicine and Surgery, College of Veterinary Medicine, University of Duhok, Duhok, Iraq | ||
| 2Department of f Medicine and Surgery, College of Veterinary Medicine, University of Duhok, Duhok, Iraq | ||
| Abstract | ||
| Cystic echinococcosis (CE) is a significant cyclo-zoonotic parasitic disease with worldwide distribution caused by larval stages (metacestodes) of Echinococcus granulosus tapeworm. The present study was conducted to determine the current prevalence, histopathology, and molecular identification of CE in sheep and goats of Duhok province, Iraq. Out of 4059 slaughtered sheep and 357 slaughtered goats, the total prevalence of hydatidosis was 9.98% and 7.56%, respectively. The liver was determined as the primary site for infection (91.44%) in both of the hosts, followed by lung (75.0%), kidney (0.69%), and spleen (0.46%). The examination of infected organs showed a moderate to severe buildup of certain immune cells around the cysts, which were either sterile, fertile, or calcified. Additional pathological findings, including fibrous tissue proliferation, necrosis, oedema, and structural collapse of tissues adjacent to the cysts were also recorded. Furthermore, molecular characterization based on PCR amplification encoding the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene confirmed the presence of Echinococcus granulosus sensu stricto (s.s) genotype (G1 and G3 strains) in isolates obtained from organs of sheep and goats. Sequencing of the obtained nucleotides revealed a high level of similarity, reaching 99.45% to 100% in compare with other GenBank retrived-sequences of G1 and G3 strains published globally. Alignment and phylogenetic analysis clustered all our sequences with G1 strain, except for a single nucleotide polymorphism in isolate PV642913 clustered with G3 strain. To the authors knowledge, this is the first molecular study to identifiy G1 and G3 strains of E. granulosus in sheep and goats in Duhok province, Iraq. | ||
| Keywords | ||
| Sheep and Goats; Histopathology; Molecular characterization; E. granulosus G1 and G3 strains | ||
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