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Assessment of Bacterial Sterilization Efficiency Using a Custom-Designed Cold Atmospheric Pressure Plasma Jet System | ||
| Rafidain Journal of Science | ||
| Articles in Press, Accepted Manuscript, Available Online from 06 October 2025 | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/rjs.2025.162556.1486 | ||
| Author | ||
| Samar Ahmed saeed* | ||
| department of physics/ collage of science/ university of mosul | ||
| Abstract | ||
| To study bacterial sterilization, a tungsten pin-to-pin cold atmospheric pressure plasma system was developed and used. Standard operating parameters, such as the DC discharge voltage, discharge current, electrode diameter, electrode spacing, ballast resistance, and airflow rate, were used to operate the system. Eleven kV, 50 µA, 0.3 mm, 11 mm, 17 kΩ, and 10 L/min were the primary working parameters. The effectiveness of cold atmospheric pressure plasma in sterilizing a range of bacterial species, including Gram-positive and Gram-negative strains, was assessed in this study. Proteus, Pseudomonas, Klebsiella pneumoniae, Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus were among the bacterial strains analyzed. For each strain, a bacterial suspension was prepared and exposed to plasma for 5, 10, 15, and 25 min. The bacterial killing ratio showed that longer exposure intervals correlated with progressive reductions in viable cell counts, culminating in complete sterilization at the maximum treatment time (25 min). The efficiency of the system in producing cold atmospheric pressure plasma was confirmed, and its potential as a dependable and safe sterilization technique was demonstrated. Sterilization efficiency was measured by counting the remaining colonies after treatment and standardizing against a 0.5 McFarland reference. | ||
| Keywords | ||
| Atmospheric pressure cold plasma; Cold plasma sterilization; sterilization | ||
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