Therapeutic role of alcoholic extract of Ephedra alata against lead acetate-induced hepatotoxicity in male albino rats

[1] Gunjan M, Naing TW, Saini RS, Ahmad A, Naidu JR, Kumar I.Marketing trends & future prospects of herbal medicine in the treatment of various disease.World Journal of Pharmaceutical Research. 2015;4(9):132-55

[2] Alamgir ANM. In: Medicinal, Non-medicinal, Biopesticides, Color- and Dye-Yielding Plants; Secondary Metabolites and Drug Principles; Significance of Medicinal Plants; Use of Medicinal Plants in the Systems of Traditional and Complementary and Alternative Medicines (CAMs). Springer International Publishing; 2017. p. 61–104.10.1007/978-3-319-63862-1 3

[3] Soumaya B, Yosra E, Rim BM, Sarra D, Sawsen S, Sarra B, et al. Preliminary phytochemical analysis, antioxidant, anti-inflammatory and anticancer activities of two Tunisian Ephedra species: Ephedra alata and Ephedra fragilis.South African Journal of Botany. 2020;135:421–428. 10.1016/j.sajb.2020.09.033

[4] Jaradat N, Hussen F, Al Ali A.Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of Ephedra alata Decne.Journal of Materials and Environmental Science. 2015;6(6):1771-8

[5] Al-Rimawi F, Abu-Lafi S, Abbadi J, Alamarneh AAA, Sawahreh RA, Odeh I. Analysis Of Phenolic And Flavonoids Of Wild Ephedra Alata Plant Extracts By Lc/Pda And Lc/Ms And Their Antioxidant Activity. African Journal of Traditional, Complementary and Alternative Medicines. 2017;14(2):130–141.10.21010/ajtcam.v14i2.14

[6] Khattabi L, Boudiar T, Bouhenna MM, Chettoum A, Chebrouk F, Chader H, et al. RP-HPLCESI-QTOF-MS Qualitative Profiling, Antioxidant, Anti-Enzymatic, Anti-Inflammatory, and NonCytotoxic Properties of Ephedra alata Monjauzeana.Foods. 2022;11(2):145.10.3390/foods11020145

[7] Tiss M, Souiy Z, Achour L, Hamden K.Ephedra alata extracts exerts anti-obesity, anti-hyperglycemia, anti-antipyretic and analgesic effects.Nutrition & Food Science. 2022;52(1):119–128.10.1108/nfs03-2021-0086

[8] Dbeibia A, Emhemmed F, Bahia W, Amri Z, Mahdhi A, Znati M, et al. Phenolic Profile with Biological Activities Assessment of Ethanolic and Aqueous Extracts from Ephedra alata.Chemistry Africa. 2024;7(5):2495–2506.10.1007/s42250-024-00940-3

[9] Zaynab M, Al-Yahyai R, Ameen A, Sharif Y, Ali L, Fatima M, et al. Health and environmental effects of heavy metals. Journal of King Saud University-Science. 2022;34(1):101653. 10.1016/j.jksus.2021.101653

[10] Balali-Mood M, Naseri K, Tahergorabi Z, Khazdair MR, Sadeghi M.Toxic mechanisms of five heavy metals: mercury, lead, chromium, cadmium, and arsenic.Frontiers in pharmacology. 2021;12:643972. 10.3389/fphar.2021.643972

[11] Raj K, Das AP. Lead pollution: Impact on environment and human health and approach for a sustainable solution. Environmental Chemistry and Ecotoxicology. 2023;5:79-85. 10.1016/j.enceco.2023.02.001

[12] Rahman MS, Ahmed Z, Seefat SM, Alam R, Islam ARMT, Choudhury TR, et al.Assessment of heavy metal contamination in sediment at the newly established tannery industrial Estate in Bangladesh: A case study.Environmental Chemistry and Ecotoxicology. 2022;4:1–12.10.1016/j.enceco.2021.10.001

[13] Singh A, Sharma A, K Verma R, L Chopade R, P Pandit P, Nagar V, et al. In: Heavy Metal Contamination of Water and Their Toxic Effect on Living Organisms. IntechOpen; 2022. 10.5772/intechopen.105075

[14] Uddin MM, Zakeel MCM, Zavahir JS, Marikar FMMT, Jahan I. Heavy Metal Accumulation in Rice and Aquatic Plants Used as Human Food: A General Review. Toxics. 2021;9(12):360. 10.3390/toxics9120360

[15] Vajargah MF, Azar H.Investigating the effects of accumulation of lead and cadmium metals in fish and its impact on human health. Journal of Aquaculture & Marine Biology. 2023;12(2):209–213. 10.15406/jamb.2023.12.00376

[16] Aslam M, Aslam A, Sheraz M, Ali B, Ulhassan Z, Najeeb U, et al. Lead toxicity in cereals: mechanistic insight into toxicity, mode of action, and management. Frontiers in plant science. 2021;11:587785.10.3389/fpls.2020.587785

[17] Kittana N, Abu-Rass H, Sabra R, Manasra L, Hanany H, Jaradat N, et al.Topical aqueous extract of Ephedra alata can improve wound healing in an animal model. Chinese Journal of Traumatology. 2017;20(2):108–113.10.1016/j.cjtee.2016.10.004

[18] Reitman S, Frankel S. In vitro determination of transaminase activity in serum. Am J Clin Pathol. 1957;28(1):56-63

[19] Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.Analytical Biochemistry. 1979;95(2):351–358.10.1016/0003-2697(79)90738-3

[20] Apak R, G¨uc¸l¨u K, ¨Ozy¨urek M, C¸elik SE. Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay.Microchimica Acta. 2007;160(4):413–419. 10.1007/s00604-007-0777-0

[21] Suvarna KS BJ Layton C.Bancroft’s theory and practice of histological techniques.Elsevier; 2019. 10.1016/c2015-0-00143-5

[22] Collin MS, Venkatraman SK, Vijayakumar N, Kanimozhi V, Arbaaz SM, Stacey RGS, et al. Bioaccumulation of lead (Pb) and its effects on human: A review.Journal of Hazardous Materials Advances. 2022;7:100094.10.1016/j.hazadv.2022.100094

[23] AL-Megrin WA, Alkhuriji AF, Yousef AOS, Metwally DM, Habotta OA, Kassab RB, et al.Antagonistic Efficacy of Luteolin against Lead Acetate Exposure-Associated with Hepatotoxicity is Mediated via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Activities. Antioxidants. 2019;9(1):10. 10.3390/antiox9010010

[24] Abdelhamid FM, Mahgoub HA, Ateya AI. Ameliorative effect of curcumin against lead acetateinduced hemato-biochemical alterations, hepatotoxicity, and testicular oxidative damage in rats. Environmental Science and Pollution Research. 2020;27(10):10950-65.10.1007/s11356-020-077183

[25] Yuniarti WM, Krismaharani N, Ciptaningsih P, Celia K, Veteriananta KD, Ma’ruf A, et al. The protective effect of Ocimum sanctum leaf extract against lead acetate-induced nephrotoxicity and hepatotoxicity in mice (Mus musculus).Veterinary World. 2021;14(1):250.10.14202/vetworld.2021.250258

[26] Srinivasan S, Murali R, Vinothkumar V, Raajasubramanian D, Narendra K, Ashokkumar N, et al. In: Therapeutic Potential of Honey Against Hepatotoxicity and Renal Toxicity: Insight into Its Mechanisms of Action. Springer Nature Singapore; 2024. p. 389–410.10.1007/978-981-97-35655 17

[27] Sioud F, Ben Toumia I, Lahmer A, Khlifi R, Dhaouefi Z, Maatouk M, et al. Methanolic extract of Ephedra alata ameliorates cisplatin-induced nephrotoxicity and hepatotoxicity through reducing oxidative stress and genotoxicity. Environmental Science and Pollution Research. 2020;27(11):12792–12801.10.1007/s11356-020-07904-3

[28] Hussein S, Abdalla mousa mohamed M, Eltawaty S, Soliman A.Phenolic Compounds From Ephedra Alata veill Growing in Libya and The Ameliorative Effect Towards CCl4-Induced Hepatotoxicity in Rats.Egyptian Journal of Chemistry. 2024;67(3):235-43.10.21608/ejchem.2023.217103.8126

[29] Chouikh A. Phytochemical Profile, Antioxidant, Analgesic And Hypolipidaemic Effects Of Ephedra Alata Decne. Female Cones Extract.FARMACIA. 2020;68(6):1011–1020.10.31925/farmacia.2020.6.7

[30] Abd allah ESH, Badary DM. Folic acid protects against lead acetate-induced hepatotoxicity by decreasing NF-kB, IL-1β production and lipid peroxidation mediataed cell injury.Pathophysiology. 2017;24(1):39–44.10.1016/j.pathophys.2017.02.002

[31] Harshitha P, Bose K, Dsouza HS.Influence of lead-induced toxicity on the inflammatory cytokines. Toxicology. 2024;503:153771.10.1016/j.tox.2024.153771

[32] Ozkaya A, Sahin Z, Dag U, Ozkaraca M.Effects of Naringenin on Oxidative Stress and Histopathological Changes in the Liver of Lead Acetate Administered Rats.Journal of Biochemical and Molecular Toxicology. 2016;30(5):243–248.10.1002/jbt.21785

[33] Haouas Z ZIHHMIMM Sallem A.Hepatotoxic Effects of Lead Acetate in Rats: Histopathological and Cytotoxic Studies.Journal of Cytology & Histology. 2014;5(5).10.4172/2157-7099.1000256

[34] Ahmed AH, Salman SS, Khaleel ZI.Histological Study On The Effect Of Different Concentrations Of Lead Acetate In The Liver, Heart And Bone Marrow In Mice (Mus Musculus).Biochemical & Cellular Archives. 2019;19(2).10.35124/bca.2019.19.2.3751

[35] Khattabi L, Chettoum A, Hemida H, Boussebaa W, Atanassova M, Messaoudi M. Pirimicarb induction of behavioral disorders and of neurological and reproductive toxicities in male rats: Euphoric and preventive effects of Ephedra alata Monjauzeana.Pharmaceuticals. 2023;16(3):402. 10.3390/ph16030402