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The effect of GnRH analogues on oocyte morphology and subsequent embryo development following intra cytoplasmic sperm injection (ICSI) | ||
| Journal of University of Anbar for Pure Science | ||
| Volume 19, Issue 2, November and December 2025, Pages 29-34 PDF (185.91 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2025.162445.1501 | ||
| Authors | ||
| Muhjah Falah Hassan* 1; Saba Sabeeh Hussain2; Rabab Zahir Al-Yasiry3; Sahbaa Hafidh Sagban4; Pallav Sengupta5; Sulagna Dutta6 | ||
| 1Department of Anatomy, Histology and Embryology, College of Medicine, University of Kerbala, Kerbala, Iraq | ||
| 2Department of Anatomy, Histology and Embryology, College of Medicine, Thiqar University, Thiqar, Iraq. | ||
| 3Department of Anatomy, Histology and Embryology, College of Medicine, Babylon University, Hilla, Iraq. | ||
| 4Department of Anatomy, Histology and Embryology, College of Medicine, Karbala University, Karbala, Iraq, | ||
| 5Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman, UAE | ||
| 6Basic Medical Sciences Department, College of Medicine, Ajman University, Ajman, UAE. | ||
| Abstract | ||
| A competent, morphologically normal gamete (oocyte or sperm) is essential for successful fertilization, the development of a good-quality embryo, and implantation. Intracytoplasmic sperm injection (ICSI) allows direct microscopic assessment of gamete quality and the study of factors that may affect gamete quality and development. The aim of this study was to assess the effect of different gonadotropin-releasing hormone (GnRH) analogue protocols (antagonist vs agonist) on oocyte maturity, morphology, quality, and embryo development. Fifty-three subfertile women were included. Participant age ranged from 18–37 years and body mass index (BMI) from 18.9–24.9 kg/m². All underwent ICSI using GnRH antagonist and agonist protocols. Microscopic evaluation of oocyte and embryo quality was performed and compared between groups. Among all parameters studied (total retrieved oocytes; oocyte quality and morphology; embryo quality; fertilization and cleavage rates), only the total number of good-quality embryos was significantly higher and the total number of poor-quality embryos was significantly lower in the GnRH antagonist group. Thus, the GnRH antagonist significantly increases the rate of developing good-quality embryos and reduces the rate of poor-quality embryos, despite producing a nonsignificantly higher number of immature and morphologically abnormal oocytes, which appear to have no impact on embryo quality or subsequent development. | ||
| Keywords | ||
| GnRH analogues; ICSI; Oocyte maturity; Oocyte morphology | ||
| References | ||
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