Ferric Ion as an Oxidizing agent for sensitive spectrophotometric Assay of Furosemide

Daoud, Shaimaa Adnan; Mahmood, Hanaa Shuker

doi:10.33899/rjs.2025.163672.1502

	Ferric Ion as an Oxidizing agent for sensitive spectrophotometric Assay of Furosemide
Rafidain Journal of Science
Articles in Press, Accepted Manuscript, Available Online from 12 November 2025
Document Type: Research Paper
DOI: 10.33899/rjs.2025.163672.1502
Authors
shaimaa adnan Daoud^* ¹; hanaa shuker Mahmood²
¹Department of Chemistry College of Science University of Mosul
²Department of Chemistry/ College of Science/ University of Mosul/ Mosul/ Iraq.
Abstract
An accurate, simple, selective, and sensitive spectrophotometric assay of furosemide (FSD) in tablets has been proposed involving an oxidation procedure. Ferric ion was used for oxidation of FSD and converted to ferrous ion, the last one reacts selectively with the organic reagent 1,10- phenanthroline to form soluble and stable red complex measured at 510 nm.The method exhibits excellent applicability for determination of FSD in tablets of Iraqi drugs company (SDI) besides the Engelland drugs company (accord). The linearity was from 2 to 15 µg/ml, and a deviation is occurred as the concentration increases over 15µg/ml with high determination coefficient 0.9919. The molar absorptivity, the LOD, LOQ 14,089.524 l.mol-1. cm-1., 0.08656 , 0.17314 respectively. The determination of FSD in Iraqi, and England tablets show high accuracy 99.63%, 99.65% *(as an average) respectively and good precision 0.5812% 0.8763% (as an average) respectively. A standard addition method show no interfering effect of other excipients.
Keywords
Furosemide; Ferric Ion; oxidation; 1; 10-phenanthrolin

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