Diarrhea is a significant cause of illness and death in many parts of the world and people of all ages. Fecal-oral transmission and intake of food and water polluted with pathogenic organisms are the major causes of acute infectious diarrhea, based on research. This investigation was carried out to isolate various bacteria that cause diarrhea in children below 5 years, and 80 stool samples were collected The study included the effect of age and area of residence on the incidence of diarrhea. These samples were planted on selective and differential culture media, which included the media of the MacConkey agar and blood agar, as well as the use of biochemical tests to diagnose these isolates using Vitek compact 2. The results of the study showed that 60 bacteria isolates belonging to seven different genera were obtained, Escherichia coli, SalmonellaTyphimurium, Klebsiella pneumonia, Shigella spp, Pseudomonas aeruginosa, Enterobacter aeruginosa, and Proteus mirabilis. And at the rates of 23.33%, 20%, 16.66%, 16.66%, 15%, 5%, and 3.33%, respectively. The results showed that the age group (1-2 years) recorded a high percentage of diarrhea. The sensitivity test showed that all the isolates were 100% sensitive to Ciprofloxacin and 100% Amoxicillin resistant, while these isolates varied in their sensitivity to the rest of the antibiotics. Finally, this investigation confirmed that diarrhea in children under the age of five was caused by multiple-drug resistant, gram-negative enterobacteria. |