Isolation and genetic diagnosis of local isolate Enterococcus faecium with evaluation of inhibitory against bacterial pathogens

Ali, Zainab A.

	Isolation and genetic diagnosis of local isolate Enterococcus faecium with evaluation of inhibitory against bacterial pathogens
Al-Kunooze Scientific Journal
Volume 11, Issue 2, April 2025, Pages 36-44 PDF (810.5 K)
Document Type: Original Article
Author
Zainab A. Ali
Abstract
Objective: Isolation the bacteria from food source has a probiotic characterization and evaluate the inhibitory activity against bacterial pathogens. Methods: Lactic acid bacteria from various sources are isolated by the MRS-CaCO3Agar medium, the isolates were purified and microscopic, biochemical were tested. The antibacterial activity of cell free supernatant (CFS) against Bacillus subtilis , Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli also testing. The isolate was selected based on high inhibitory activity and genetic diagnosis were performed. Results: the highest inhibitory diameter against Gram-positive bacteria was recorded with a diameter of 13 mm to Staph aureus. The results showed that the bacterial isolate Gram positive, immobile, negative for catalyze, Esculin hydrolysis, and CO2 production from glucose, grows at 10- 45⸰C, 2- 6.5% Nacl, and at pH 4.5- 6.9. It had the ability to ferment of sucrose, mannitol, arabinose, sorbitol and xylose. The diagnosis of isolation has been genetically confirmed to the species Enterococcus faecium. Conclusion: The E. faecium can be considered as probiotics in a safe manner to adaptive immune, in addition to the possibility of using it as a potential source of natural antibacterial agent in food and pharmaceutical industries.
Keywords
Enterococcus faecium; PCR; Antibacterial; pathogens

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