Direct sequence of D13S317 loci for analysis and identification of STR structure in Iraqis

Khudheyer Derraj Almyahi, Maysoon

	Direct sequence of D13S317 loci for analysis and identification of STR structure in Iraqis
Al-Kunooze Scientific Journal
Volume 11, Issue 4, October 2025, Pages 52-62 PDF (362.42 K)
Document Type: Original Article
Author
Maysoon Khudheyer Derraj Almyahi
Abstract
The analysis of DNA sequences plays a critical role in genetic research, but challenges persist in attaining high precision with mixed sequences. This study aims to evaluate the efficiency of the Mixed Sequence Analysis application in analyzing DNA sequences with heterozygous base calling as well as manual analysis. DNA was extracted from 31 unrelated Iraqi individuals, and D13S317 is one specific STR locus that is highly variable among individuals and was amplified. The mixed sequence was then analyzed using the Mixed Sequence Analysis tool, complemented by manual chromatogram interpretation. The most frequent allele detected was TATC 12 (52.27%), followed by TATC 9 (20.45%). The least frequent allele was TATC10, which was 4.55%; also, different alleles were detected with high variability. The analysis revealed that while the application was beneficial, there were challenges in distinctive variations at the base level due to noise in the chromatogram. Despite its limitations, this research highlights the importance of developing accurate sequencing analysis techniques, particularly in situations where other methods are unavailable.
Keywords
Genetic Variation; STR; D13S317 Locus; DNA sequencing

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