- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Comparative analysis of IL-2, IL-6, and IFN-γ responses in relation to human and animal Rotavirus VP7 Gene | ||
| Al-Kunooze Scientific Journal | ||
| Volume 11, Issue 4, October 2025, Pages 169-178 PDF (734.6 K) | ||
| Document Type: Original Article | ||
| Author | ||
| Rawaa Bannay Zubairi , Basil A. Abbas* , Fawziah Ali Abdullah | ||
| Abstract | ||
| Rotavirus infection is considered to be one of the zoonotic diseases, transmitted between humans and animals. The present study aimed to investigate the association between cytokine (IL-2, IL-6 and INF-γ) levels with Rotavirus VP7 gene distribution in humans and cattle by using Polymerase Chain Reaction (PCR) and Enzyme Linked Immunosorbent Assay (ELISA). Faecal samples (54) and 54 blood samples were collected from each diarrheic human and cattle. The PCR-VP7 results revealed that VP7-PCR positivity was observed in 12 human stool(22.2%) and 14 cattle faecal samples (25.9%). Non-significant effect (P>0.05) for human sex on Rota virusVP7 distribution, and (23.3%) of Rota virusVP7 detection was observed in males compared to females (20.8%). Significant effect (P<0.05) for cattle sex on Rota virus VP7 distribution, and a higher percentage (47.1%) of Rota virus VP7 infection in cattle was observed in females compared to males (16.2%). Non-significant effect (P>0.05) for human and cattle age was observed in this study. A higher percentage of Rota virus VP7 infection was observed in humans and cattle (23.5 and 34.6 % respectively) in the age group less than 5 years. ELISA-based IL-6, IL-2 and INF-γ results revealed that Positive cases with VP7 did not differ significantly by species, age, or sex. among the measured cytokines, only IL-6 in cattle showed a significant elevation in VP7-positive cases compared with VP7-negative controls (356.87±156.13 vs. 40.84±40.84 pg/mL, p = 0.020). | ||
| Keywords | ||
| Rotavirus A; Comparative Analysis; cattle; humans; VP7 gene, conventional RT- PCR, cytokines | ||
|
Statistics Article View: 124 PDF Download: 50 |
||