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Cytotoxicity of Chitosan Nanoparticles_Incorporated with Different Endodontic Irrigation Solutions (An in Vitro Study) | ||
| Tikrit Journal for Dental Sciences | ||
| Volume 13, Issue 2, December 2025, Pages 384-389 PDF (334.09 K) | ||
| DOI: 10.25130/tjds.13.2.10 | ||
| Authors | ||
| Sarah Flaah Ali* 1; Fanar T.A. Al-jadwaa2; Suhad Mwafaq Hamdoon3 | ||
| 1Department of Conservative Dentistry, College of Dentistry, University of Mosul, Mosul, Iraq. | ||
| 2Department of Conservative Dentistry, College of Dentistry, University of Mosul, Mosul, Iraq | ||
| 3Department of Dental Basic Sciences, College of Dentistry, University of Mosul, Mosul, Iraq | ||
| Abstract | ||
| Aim: The investigation was done to evaluate the cytotoxicity of two concentrations of chitosan-nanoparticles (0.2% ,0.5%) _incorporated with sodium hypochlorite (5.25%) and chlorhexidine (2%) at different time periods. Methods: For evaluating cytotoxicity, human periodontal fibroblast cell lines were exposed to different endodontic irrigation solutions. Cytotoxicity was assessed immediately, after 24 hours, after 72 hours and after 168 hours, using Methyl Thiazol Tetrazolium (MTT) assay. Result: According to the result of this study, sodium hypochlorite 5.25% was the most cytotoxic solution (lower cell viability) followed by chlorhexidine 2%, nano-chitosan 0.5% while nano-chitosan 0.2% was the lowest cytotoxic irrigation solution (higher cell viability). After mixing of sodium hypochlorite and chlorhexidine with nanoparticles we noticed that incorporating the nanoparticles lowers the cytotoxicity of these irrigation solutions. Conclusion: This research demonstrates that chitosan nanoparticles can improve cytotoxicity of 5.25% sodium hypochlorite and 2% chlorhexidine when they mixed together. | ||
| Keywords | ||
| Endodontic irrigation; Nanochitosan; Cytotoxicity; MTT assay | ||
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