- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Molecular characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis and human contacts in Jordan | ||
| Iraqi Journal of Veterinary Sciences | ||
| Volume 40, Issue 2, April 2026, Pages 225-234 PDF (816.99 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/ijvs.2025.165580.4532 | ||
| Authors | ||
| Mohammad H, Gharaibeh* 1; Luay Abu-Qatouseh2; Ismail Malkawi3; Adi Nawasreh4; Abdallah Al Dwekat4; Mohammed Alkhouraki4; Zeinab Arabeyyat5; Gamal Wareth6, 7; Amir Abdulmawjood8 | ||
| 1Faculty of Veterinary Medicine Jordan University of Science and Technology P.O. Box 3030, Irbid 22110, Jordan | ||
| 2Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy, University of Petra, Amman, Jordan | ||
| 3Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan | ||
| 4Department of Basic Veterinary Medical Science, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan | ||
| 5The University of Jordan, Aqaba branch, (Department of Marine Biology), Aqaba, Jordan | ||
| 6Friedrich-Loeffler-Institut Bundesforschungsinstitut für Tiergesundheit Federal Research Institute for Animal Health Naumburger Str. 96a | ||
| 707743 Jena | ||
| 8Department of Foodborne Zoonoses Institute of Food Quality and Food Safety University of Veterinary Medicine Hannover Bischofsholer Damm 15 30173 Hannover | ||
| Abstract | ||
| Methicillin-resistant Staphylococcus aureus (MRSA) infection has a significant impact on public health. Therefore, this study investigates the occurrence of MRSA isolated from cows with subclinical mastitis in Jordan during the period from January 2021 to December 2023. It is also to compare their genotypic and phenotypic characteristics with those of isolates from their associated human strains. To achieve this objective, milk samples were collected aseptically and cultured on mannitol salt agar and blood agar. The isolates were identified based on colony morphology, biochemical tests, and PCR confirmation targeting the nuc and mecA genes. In addition, molecular-based typing techniques (MLST and spa typing) have been applied and supplemented by antimicrobial susceptibility testing. A total of 310 bovine S. aureus isolates were identified, of which 52 (16.8%) were MRSA. Besides that, 49 and 33 MRSA strains were isolated from people related to and unrelated to these animals, respectively. Antimicrobial susceptibility testing revealed high resistance rates to β-lactams, macrolides, and tetracyclines. Nine distinct spa clusters and 21 different spa types were observed. The most prevalent spa type among the isolates was t386 (38%, n=17). For the first time, two novel spa types were discovered among the isolates examined in this study. The cluster pattern of spa isolates indicates a possible close relationship between human and animal isolates. | ||
| Keywords | ||
| MRSA; Bovine mastitis; Zoonotic transmission; MLST; and Spa typing | ||
|
Statistics Article View: 1,194 PDF Download: 474 |
||