Bioactive Metabolites in a New Strain of Streptomyces griseus RH250 from Marl Clay in Mosul/Iraq

Khalaf, Rawaa A.; Altai, Hiyam Adel

doi:10.33899/rjs.2025.190521

	Bioactive Metabolites in a New Strain of Streptomyces griseus RH250 from Marl Clay in Mosul/Iraq
Rafidain Journal of Science
Volume 34, Issue 4, December 2025, Pages 48-58 PDF (642.62 K)
Document Type: Research Paper
DOI: 10.33899/rjs.2025.190521
Authors
Rawaa A. Khalaf^* ¹; Hiyam Adel Altai^* ²
¹Department of Biology/ College of Science/ University of Mosul/ Mosul
²Biology College of Science University of Mosul Mosul/Iraq
Abstract
Identification of the bioactive secondary metabolites of a crude butanol extract from a locally isolated strain of Streptomyces griseus RH250 isolated from marl clay in Mosul/ Iraq. This new strain possesses the ability to produce various kinds of metabolites, it was screened for it is antibacterial activity against Gram-negative (Alcaligenes faecalis, Pseudomonas fluorescens, Serratia marcescens, and Escherichia coli) and Gram-positive (Staphylococcus aureus). Screening was performed using the agar-cross streak method, and the growth inhibition line was measured in millimeters. This strain has shown potent in vitro antibacterial activities against Staphylococcus aureus (25 mm) only. The agar diffusion well technique for crude n-butanol extract was performed. Gas chromatography-mass-mass spectrometry was used to determine bioactive secondary metabolites in crude butanol extract. This analysis showed that twenty-one bioactive metabolite compounds were isolated from this strain, which have different chemical structures and biological activities.
Keywords
Biological activity; cross streak; crude butanol extract; Streptomyces griseus RH250

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