Spectrophotometric Estimation of Levofloxacin Via Ion-Pair Complex Formation with Erythrosine B.

Al-nouaemy, Dheyaa Thamer; Omer, Khalida Mohammed

doi:10.33899/rjs.2025.190524

	Spectrophotometric Estimation of Levofloxacin Via Ion-Pair Complex Formation with Erythrosine B.
Rafidain Journal of Science
Volume 34, Issue 4, December 2025, Pages 79-89 PDF (675.28 K)
Document Type: Research Paper
DOI: 10.33899/rjs.2025.190524
Authors
Dheyaa thamer Al-nouaemy^* ¹; Khalida Mohammed Omer^* ²
¹Department of Chemistry/ College of Science/ University of Mosul/ Iraq
²Dept. of Chem., college of science, Mosul Univerity
Abstract
In this study, a simple, rapid, and cost-effective analytical method was described for the quantitative determination of levofloxacin (LEV) in its pure state and in therapeutic doses. The method relies on forming an ion-pair complex between LEV and erythrosine dye (ERY) at pH=4.0. The spectrum of the coloured product exhibits maximum absorption at 558 nm against the blank. A linear relationship, was obtained in the concentration range of 1.0-15.0 μg/ml with a quantification coefficient of 0.9991 under the optimum conditions. The molar absorption coefficient and Sandell’s sensitivity index values were 1.91×104 L/mol.cm and 0.0189 μg/cm² respectively, whereas the values of the detection limit and the quantification limit were 0.200 and 0.666 μg/ml respectively. The developed method was effectively applied to estimate LEV present in pharmaceutical dosage. The accuracy and reliability of the suggested method were ascertained by recovery study via the standard addition method.
Keywords
Levofloxacin; ion-pair complexation; determination; spectrophotometric; erythrosine
Supplementary Files البحث التاسع ضياء وخالدة منسق.pdf
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