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Biosynthesis of Silver Nanoparticles From Schizophyllum commune: Extraction Via HPLC Structural Profiling by GC-MAS and Assessment of Antimicrobial Eifficancy Against Some Pathogenic Bacteria and Fungi. | ||
| Rafidain Journal of Science | ||
| Articles in Press, Accepted Manuscript, Available Online from 15 December 2025 | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/rjs.2025.165938.1524 | ||
| Authors | ||
| Shifaa Tayyar AL-Assaf* 1; Faten Mula Abed2 | ||
| 1Department of Biology, College of Education for Women, University of Mosul, Mosul, Iraq | ||
| 2Department of Biology, College of Science, University of Mosul, Mosul, Iraq | ||
| Abstract | ||
| This study show identification of antibacterial and antifungal properties of )AgNPs( produced by filamentous fungus strain Schizophyllum commune. Although there are literatures reporting on successful biosynthesis of Ag NPs, however biosynthesis of Ag NPs using fungi strains is limited., particularly of natural origin such as fungi. Basidiomycetes are large source of bioactive secondary metabolites with high potential for therapy. Schizophyllum commune a basidiomycete fungus isolated from natural sources in Mosul, Iraq. And adding N-L-acetyl-cystine on extraction and use Gas Chromatography-Mass Spectrometry (GC-MS) analysis detected bioactive compounds: silver benzoate Later, High-Performance Liquid Chromatography (HPLC) confirmed the high percentage of compound in fungal extract, then Scanning electron microscopy (SEM) characterization of the silver benzoate-derived synthesized nanomaterial determines its intricate morphology, particle size, and distribution, which are important to evaluate its functionality. The antimicrobial activity was measured by microplate assay against clinically significant pathogens Escherichia coli, Staphylococcus aureus, Bacillus cereus Pseudomonas fluorescens, Candida albicans, and Aspergillus niger. Silver benzot was more inhibitory to all microbes growth rates, Benzoic acid-silver salt shown potent antibacterial action against B. cereus and P. fluorescens, while its activity against fungi strains was less effective., antimicrobial activity. Findings establish Schizophyllum commune as source of novel antimicrobial molecules of pharmacological interest. The combination of biochemical characterization and antimicrobial screening provides a sound basis for continuing research on the discovery of new medicines with activity against drug-resistant pathogens. Additional work toward in vivo validation and identification of mechanism-based pathways is warranted to fully determine the therapeutic potential of such fungal metabolites. | ||
| Keywords | ||
| Silver nanoparticles; biosynthesis of particles; Schizophyllum commune; microplate assay | ||
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